Figure 3: In silico analysis of messenger RNA structure and stability of c.816C>T (p.L272L) in parkin RBR E3 ubiquitin protein ligase (PRKN) using Mfold. The mRNAsecondary structure is stabilized by hydrogen bonds, represented by three different colours;AU blue, CG red and GU green. The nucleotide position has been indicated by a small asterisk and the regions altered in mutant are indicated by arrows. Inset showing part of image in enlarged form. (A) Wild type and (B) Mutant.