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  Indian J Med Microbiol
 

Figure 3: Flow cytometric analysis of CD4+CD25+FOXP3+cells. (A) Lymphocytes are gated in forward light scatter (FS) vs. side light scatter (SS) dot plot, then (B) CD3+ cells gated in CD3- E610 vs. SS, followed by (C) CD4+ gating in CD3- E610 vs. CD4- FITC. (D) CD4+ cells gated are plotted CD25-PC5 vs. FoxP3-PE for assessing CD4+CD25+FoxP3+ (C2) cell expression.

Figure 3:  Flow cytometric analysis of CD4<sup>+</sup>CD25<sup>+</sup>FOXP3<sup>+</sup>cells. (<b>A</b>) Lymphocytes are gated in forward light scatter (FS) vs. side light scatter (SS) dot plot, then (<b>B</b>) CD3+ cells gated in CD3- E610 vs. SS, followed by (<b>C</b>) CD4+ gating in CD3- E610 vs. CD4- FITC. (<b>D</b>) CD4+ cells gated are plotted CD25-PC5 vs. FoxP3-PE for assessing CD4+CD25+FoxP3+ (C2) cell expression.