Figure 5: (A) Significant (*P <0.05) expression of collagen type II (coll II), particularly in high-density seeded cells. All evaluated genes showed progressive expression throughout the culture period. Constructs showed strong expression of collagen type II in the pericellular matrix area and throughout the extracellular matrix, confirming an immature cartilage phenotype at days 14 and 21 for both groups glyceraldehyde phosphate dehydrogenase (GAPDH) and glycosaminoglycan (GAG) (B). Proteoglycan deposition showed no significant differences between constructs (C&D). Immunohistochemistry staining. Collagen type I and II (coll I & II) deposition was also detected in both constructs at day 7, 14 and 21 in low and high seeding chondrocytes fibrin construct.