Figure 2: (A). Photomicrographs (×40) of human osteoarthritis chondrocytes at day 7 from P0, P1, P2 and P3. After seven days of in vitro culture, all chondrocytes demonstrated polygonal morphology. (B) The growth rate of cultured chondrocytes decreased over successive passages *P <0.05. (C) Gene expression for collagen types I and II reduced after several passages, whereas aggrecan gene expression was consistently expressed. (D) Prominent staining of collagen type II in P0 detected by immunocytochemistry, which became weaker by P3. Collagen type I staining was observed for all passages.