Figure 3: (A) Sandwich ELISA showing limit of detection of recombinant Shiga toxin B (rStxB) using rabbit and mouse antiserum used for capture and revealing, respectively. Graph represents concentration (ng/ml) of rStxB (hollow rectangular) and negative control (solid rectangular). Cut-off value was 0.3. (B) Sandwich ELISA for detection of Shiga toxin from culture lysates. Graph represents reactivity of recombinant Shiga toxin B antiserum with rStxB, Shiga like toxin producing Escherichia coli(E. coli)(STEC), Shigella dysenteriae type 1, E. coli (SG12009) and Phosphate buffered saline (PBS).