Close
  Indian J Med Microbiol
 

Figure 3: (A) Sandwich ELISA showing limit of detection of recombinant Shiga toxin B (rStxB) using rabbit and mouse antiserum used for capture and revealing, respectively. Graph represents concentration (ng/ml) of rStxB (hollow rectangular) and negative control (solid rectangular). Cut-off value was 0.3. (B) Sandwich ELISA for detection of Shiga toxin from culture lysates. Graph represents reactivity of recombinant Shiga toxin B antiserum with rStxB, Shiga like toxin producing Escherichia coli(E. coli)(STEC), Shigella dysenteriae type 1, E. coli (SG12009) and Phosphate buffered saline (PBS).

Figure 3: (A) Sandwich ELISA showing limit of detection of recombinant Shiga toxin B (rStxB) using rabbit and mouse antiserum used for capture and revealing, respectively. Graph represents concentration (ng/ml) of rStxB (hollow rectangular) and negative control (solid rectangular). Cut-off value was 0.3. (B) Sandwich ELISA for detection of Shiga toxin from culture lysates. Graph represents reactivity of recombinant Shiga toxin B antiserum with rStxB, Shiga like toxin producing <i>Escherichia coli</i>(<i>E. coli</i>)(STEC), <i>Shigella dysenteriae</i> type 1, <i>E. coli</i> (SG12009) and Phosphate buffered saline (PBS).