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  Indian J Med Microbiol
 

Figure 2: (A) Western blot showing titre of rabbit anti-recombinant Shiga toxin B serum. Lane 1: pre-stained molecular weight marker (kDa); lanes 2-11: two-fold serial dilutions (1:1000 to 1:512,000) of anti-recombinant Shiga toxin B rabbit serum. (B) ELISA showing titre of rabbit-anti-recombinant Shiga toxin B serum. Graph showing two-fold serial dilutions of anti-recombinant Shiga toxin B rabbit serum. Blue line represents interaction of recombinant Shiga toxin B with antiserum; cut-off value is 0.15. (C) Western blot showing titre of mouse anti-recombinant Shiga toxin B serum. Lane 1: pre-stained molecular weight marker (kDa); lanes 2-11: two-fold serial dilutions (1:1000 to 1:512000) of anti-recombinant Shiga toxin B mouse serum. (D) ELISA showing titre of mouse anti-recombinant Shiga toxin B serum. Graph represents two-fold serial dilutions of anti-recombinant Shiga toxin B mouse serum; blue line represents interaction of recombinant Shiga toxin B with antiserum. Cut-off value is 0.12.

Figure 2: (A) Western blot showing titre of rabbit anti-recombinant Shiga toxin B serum. Lane 1: pre-stained molecular weight marker (kDa); lanes 2-11: two-fold serial dilutions (1:1000 to 1:512,000) of anti-recombinant Shiga toxin B rabbit serum. (B) ELISA showing titre of rabbit-anti-recombinant Shiga toxin B serum. Graph showing two-fold serial dilutions of anti-recombinant Shiga toxin B rabbit serum. Blue line represents interaction of recombinant Shiga toxin B with antiserum; cut-off value is 0.15. (C) Western blot showing titre of mouse anti-recombinant Shiga toxin B serum. Lane 1: pre-stained molecular weight marker (kDa); lanes 2-11: two-fold serial dilutions (1:1000 to 1:512000) of anti-recombinant Shiga toxin B mouse serum. (D) ELISA showing titre of mouse anti-recombinant Shiga toxin B serum. Graph represents two-fold serial dilutions of anti-recombinant Shiga toxin B mouse serum; blue line represents interaction of recombinant Shiga toxin B with antiserum. Cut-off value is 0.12.