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  Indian J Med Microbiol
 

Figure 6: Hoechst 33258 staining: Apoptotic bodies are visualized in HeLa cells. Cells were treated with siRNA-2 loaded chitosan coated PLGA nanoparticle, naked siRNA-2 and blank nanoparticle. After 48 h incubation, cells were stained with Hoechst 33258 DNA staining and morphological changes were observed using fluorescence microscope (20×). (A) siRNA2 loaded chitosan coated polylactic-co-glycolic acid nanoparticle-treated cells; (B) Naked siRNA2-treated cells; (C) Blank nanoparticle-treated cells; (D) Untreated cells.

Figure 6: Hoechst 33258 staining: Apoptotic bodies are visualized in HeLa cells. Cells were treated with siRNA-2 loaded chitosan coated PLGA nanoparticle, naked siRNA-2 and blank nanoparticle. After 48 h incubation, cells were stained with Hoechst 33258 DNA staining and morphological changes were observed using fluorescence microscope (20×). (A) siRNA2 loaded chitosan coated polylactic-co-glycolic acid nanoparticle-treated cells; (B) Naked siRNA2-treated cells; (C) Blank nanoparticle-treated cells; (D) Untreated cells.