Figure 2. Comparison of polycomb repressive complexes (PRC)expression in pancreatic progenitors derived from human embryonic stem (hES) cells on day 16 and adult human pancreas. Expression of (A) PRC1 (RING1A, RING1B, BMI1 and CBX2) and (B) PRC2 (SUZ12, EZH2, EED and JARID2) complexes was compared by qRT-PCR. Results revealed distinct epigenetic differences of pancreatic progenitors derived from hES cells and adult human pancreas when compared to that of undifferentiated human ES cells used as reference sample; analyzed by CtΔ Δ method. Expression of polycomb group (PcG) proteins in adult human pancreas (normalized to 18S, Ct40) was shown by our group earlier (Ref. 23). These differences may ameliorate after transplantation or possibly are the underlying cause to explain that hES cells differentiate into their fetal counterparts and may thus not be very useful to regenerate adult pancreas. Results are representative of five biological replicates. [Source: Adapted from Ref. 23, Figures 6 & 9, reproduced with permission].