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  Indian J Med Microbiol
 

Figure 1: Representative photograph of the 2.0 per cent agarose gel electrophoresis showing PCR products of Rabha samples, analyzed for the presence of the codon 26 mutation. Primers for mutant alleles were used for lanes 1-6 and normal primers were used for lanes 7-9 and 11-13. Lane 1: positive control for the mutant allele; lane 2: negative control; lane 3: non template control; lane 4: HbE heterozygote; lane 5: HbE homozygote; lane 6: HbE heterozygote; lane 7: positive control for normal primer; lane 8: negative control for the normal allele; lane 9: non template control; lane 10: 100 bp DNA ladder; lane 11: HbE heterozygote; lane 12: HbE homozygote; lane 13: HbE heterozygote;. The PCR product size for the internal control band (PAH) and HbE-specific band were 182 and 303 bp, respectively.

Figure 1: Representative photograph of the 2.0 per cent agarose gel electrophoresis showing PCR products of <i>Rabha</i> samples, analyzed for the presence of the codon 26 mutation. Primers for mutant alleles were used for lanes 1-6 and normal primers were used for lanes 7-9 and 11-13. Lane 1: positive control for the mutant allele; lane 2: negative control; lane 3: non template control; lane 4: HbE heterozygote; lane 5: HbE homozygote; lane 6: HbE heterozygote; lane 7: positive control for normal primer; lane 8: negative control for the normal allele; lane 9: non template control; lane 10: 100 bp DNA ladder; lane 11: HbE heterozygote; lane 12: HbE homozygote; lane 13: HbE heterozygote;. The PCR product size for the internal control band (PAH) and HbE-specific band were 182 and 303 bp, respectively.