Figure 4: (a) Representative partial metaphase spreads of B/CMBA.Ov cells exposed to MIC. (i) Normal (control) chromosomes; (ii-iv) Cells treated with constant 0.005 μM dose of MIC showing chromatid break (cb), Robertsonian (Rb) fusion, dicentric (dic), ring (r), acentric chromosomes (ac), quadriradial (quadrirad), fragments (fr) respectively. (b) Immuno-FISH of telomere repeat binding factor-2 (TRF2) protein. Microphotographs (x 600) showing telomeric localization of TRF2 protein. TRF2 expression signals were observed at the ends of metaphase chromosomes stained with the anti-TRF-2 antibody (Cyanin-3 yellow) in control cells, while treated cells showing comparatively less or no signals of TRF2 at telomeres (small white arrows). DNA was counterstained with DAPI (blue). (c) Spectral karyotyping (SKY) analysis of B/CMBA.Ov cells. Photomicrographs showing translocations (white arrows) after treatment with 0.005 μM MIC alone at 96 h (SKY view and Inverted DAPI ideogram). Whereas untreated control cells display normal metaphase with absence of cryptic translocations (SKY view and Inverted DAPI ideogram). Notably, quadri-radial formation due to translocation of chromosomes 1, 3 and 11(t1q; 3q; 11q) and also numerical gain of chromosome 15 and X chromosome with loss of chromosome 7 were observed in SKY view of treated cells.