Figure 1: Northern blot analysis of LHR mRNA in human granulosa cells immediately after retrieval (D1) and after 4 days of incubation in serum free media (D4).Total RNA was extracted from granulosa cells from day 1 and four days of incubation (D4) to recover from downregulation. RNA was separated on agarose-formaldehyde gel, transferred to nitrocellulose membranes, hybridized with the 32P-labelled hLHRcDNA, and exposed to x-ray film. To monitor RNA loading, the blot was stripped and rehybridized with radiolabelled cDNA for 18S rRNA. The blot shown is one representative of three experiments with similar results. (Source: Ref. 16, Reproduced with permission).