Figure 3. Association of HPV16 physical state with the viral load in different precancer and cancer cases. Distribution of normalized HPV16 E2:E6 ratio and HPV 16 viral load in cervical precancer (LSIL and HSIL) and cancer cases. Each circle indicates individual case. Normalized E2:E6 ratios and viral load were calculated as described in Methods. Normalized E2:E6 = 0 represents completely integrated HPV16; E2:E6 = 1 represents episomal viral genome, E2:E6 between 0 and 1 indicates concomitant HPV16 genome. Vertical red line and horizontal blue line represent median values of E2:E6 ratio and HPV16 viral load in each disease group, respectively.