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  Indian J Med Microbiol
 

Figure 4. PCR detection of Leishmania infection in mice. BALB/c mice were infected with ~1×107 promastigotes/ml of L. donovani. After 3 wk post-infection liver and spleen samples were taken out under sterile conditions, DNA was isolated and subjected to PCR analysis for confirmation of infection. Mice injected with normal saline were served as negative control. Lane 1 - Positive control (KE-16 strain DNA), lane 2 - PCR product from liver tissue sample of saline infected mice (healthy mice), lane 3 - PCR product from liver tissue sample of Leishmania infected mice (challenged mice), lane 4 - PCR product from spleen tissue sample of Leishmania infected mice (challenged mice), lane 5 - Blank (distilled water), and lane 6 - 1 Kb molecular weight marker

Figure 4. PCR detection of <i>Leishmania</i> infection in mice. BALB/c mice were infected with ~1×107 promastigotes/ml of <i>L</i>. <i>donovani</i>. After 3 wk post-infection liver and spleen samples were taken out under sterile conditions, DNA was isolated and subjected to PCR analysis for confirmation of infection. Mice injected with normal
saline were served as negative control. Lane 1 - Positive control (KE-16 strain DNA), lane 2 - PCR product from liver tissue sample of saline infected mice (healthy mice), lane 3 - PCR product from liver tissue sample of <i>Leishmania</i> infected mice (challenged mice), lane 4 - PCR product from spleen tissue sample of <i>Leishmania</i> infected mice (challenged mice), lane 5 - Blank (distilled water), and lane 6 - 1 Kb molecular weight marker