Figure 5: Enhanced transfection efficacy of retrovirus transfected dendritic cells by prolonged centrifugation. DCs were infected with EGFP shuttled retrovirus vector and green fluorescence was measured by FACS. Cells were compensated for each staining (CD11c and EGFP) and number of CD11c positive EGFP expressing cells is given. Kinetic study of CD11c expressing DCs was done from day 8 to day 12. Data show the mean fluorescence intensity with time.