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  Indian J Med Microbiol
 

Figure 4: A: Shows the expression of recombinant SSP2 protein (34 kD protein) after PCR synthesis by agarose gel electrophoresis. It was detected as 34 kD protein. Protein expression is shown in soluble and insoluble lysates of transfected DCs, transfected RL tumour cells as a control, non-transfected DCs and non-transfected RL tumour cells. Tumour cell line is also used in this experiment for the comparison among different types of antigen presenting cells. Synthesized SSP2 protein was used as the positive control. M, Marker; L, SSP2 injected line. Figure 4: B: CTLs from BALB/c mice were analyzed for its cytolytic activity of the matched antigen-pulsed target cells by 51Cr-release assay. For antigen specific CTL induction, CTLs were cultured with A and C peptides for 7 days. For cytolytic assay, CTLs were recultured with peptide-pulsed and transfected DCs and transfected P815 tumour target cells and mock transfected for the analysis for Cr-release. These antigens specific CTLs identified and killed significantly higher number of peptide transfected targets than the pulsed targets. Cytolytic activity of transfected target cells is significantly more than the RL♂ mock cells. Data represent one of the three experiments.

Figure 4: A: Shows the expression of recombinant SSP2 protein (34 kD protein) after PCR synthesis by agarose gel electrophoresis. It was detected as 34 kD protein. Protein expression is shown in soluble and insoluble lysates of transfected DCs, transfected RL tumour cells as a control, non-transfected DCs and non-transfected RL tumour cells. Tumour cell line is also used in this experiment for the comparison among different types of antigen presenting cells. Synthesized SSP2 protein was used as the positive control. M, Marker; L, SSP2 injected line.
Figure 4: B: CTLs from BALB/c mice were analyzed for its cytolytic activity of the matched antigen-pulsed target cells by 51Cr-release assay. For antigen specific CTL induction, CTLs were cultured with A and C peptides for 7 days. For cytolytic assay, CTLs were recultured with peptide-pulsed and transfected DCs and transfected P815 tumour target cells and mock transfected for the analysis for Cr-release. These antigens specific CTLs identified and killed significantly higher number of peptide transfected targets than the pulsed targets. Cytolytic activity of transfected target cells is significantly more than the RL♂ mock cells. Data represent one of the three experiments.