Year : 2017 | Volume
: 145 | Issue : 6 | Page : 746--752
Combination of interleukin-10 gene promoter polymorphisms with HLA-DRB1*15 allele is associated with multiple sclerosis
Majid Shahbazi1, Javad Sadeghi Allah Abadi1, Danial Roshandel1, Maryam Koochaki1, Hosein Amiri2, Rahim Kohansal2, Seied Mohammad Baghbanian3, Mahdi Zamani4
1 Medical Cellular and Molecular Research Center, Gorgan, Iran
2 Medical Cellular and Molecular Research Center; Department of Neurology, 5th Azar Hospital, Golestan University of Medical Sciences, Gorgan, Iran
3 Department of Neurology, Mazandaran University of Medical Sciences, Sari, Iran
4 Department of Neurogenetics, ian Centre of Neurological Research, Tehran, Iran
Background & objectives: Multiple sclerosis (MS) is common in some ethnic groups. Interleukin-10 (IL-10) is a potent anti-inflammatory and immunosuppressive cytokine that may be an important regulator in MS disease pathogenesis. IL-10 promoter includes several single nucleotide polymorphisms and the level of IL-10 expression is related to these polymorphisms. Furthermore, loci within the histocompatibility regions are responsible for susceptibility to MS. The aim of this study was to investigate the association of IL-10 gene promoter polymorphisms and HLA-DRB1*15 allele frequencies with MS susceptibility in an Iranian population.
Methods: In this study 336 MS patients and 454 healthy controls were included. Genomic DNA was purified from peripheral blood samples by a standard protocol. Genotyping was performed by the sequence-specific primer polymerase chain reaction method.
Results: IL-10 −1082 G/G and IL-10 −819 C/C genotypes were more frequent in MS patients than healthy individuals. DRB1*15 allele showed a higher frequency among MS patients compared to controls.
Interpretation & conclusions: The IL-10 and HLA-DRB1*15 polymorphisms were associated with the susceptibility to MS in Iranian patients. Our results suggest that gene-gene interaction of IL-10 polymorphisms and HLA-DRB1*15 alleles may be important factors in the development of MS.
Medical Cellular & Molecular Research Center, Golestan University of Medical Sciences, Gorgan
|How to cite this article:|
Shahbazi M, Abadi JS, Roshandel D, Koochaki M, Amiri H, Kohansal R, Baghbanian SM, Zamani M. Combination of interleukin-10 gene promoter polymorphisms with HLA-DRB1*15 allele is associated with multiple sclerosis.Indian J Med Res 2017;145:746-752
|How to cite this URL:|
Shahbazi M, Abadi JS, Roshandel D, Koochaki M, Amiri H, Kohansal R, Baghbanian SM, Zamani M. Combination of interleukin-10 gene promoter polymorphisms with HLA-DRB1*15 allele is associated with multiple sclerosis. Indian J Med Res [serial online] 2017 [cited 2021 Apr 21 ];145:746-752
Available from: https://www.ijmr.org.in/article.asp?issn=0971-5916;year=2017;volume=145;issue=6;spage=746;epage=752;aulast=Shahbazi;type=0