Year : 2015 | Volume
: 142 | Issue : 6 | Page : 747--758
Culture & differentiation of mesenchymal stem cell into osteoblast on degradable biomedical composite scaffold: In vitro study
Krishan G Jain1, Sujata Mohanty1, Alok R Ray2, Rajesh Malhotra3, Balram Airan4
1 Stem Cell Facility, All India Institute of Medical Sciences, New Delhi, India
2 Centre for Biomedical Engineering, IIT-Delhi, New Delhi, India
3 Department of Orthopedics, All India Institute of Medical Sciences, New Delhi, India
4 Department of CTVS, All India Institute of Medical Sciences, New Delhi, India
Background & objectives: There is a significant bone tissue loss in patients from diseases and traumatic injury. The current autograft transplantation gold standard treatment has drawbacks, namely donor site morbidity and limited supply. The field of tissue engineering has emerged with a goal to provide alternative sources for transplantations to bridge this gap between the need and lack of bone graft. The aim of this study was to prepare biocomposite scaffolds based on chitosan (CHT), polycaprolactone (PCL) and hydroxyapatite (HAP) by freeze drying method and to assess the role of scaffolds in spatial organization, proliferation, and osteogenic differentiation of human mesenchymal stem cells (hMSCs) in vitro, in order to achieve bone graft substitutes with improved physical-chemical and biological properties.
Methods: Pure chitosan (100CHT) and composites (40CHT/HAP, 30CHT/HAP/PCL and 25CHT/HAP/PCL scaffolds containing 40, 30, 25 parts per hundred resin (phr) filler, respectively) in acetic acid were freeze dried and the porous foams were studied for physicochemical and in vitro biological properties.
Results: Scanning electron microscope (SEM) images of the scaffolds showed porous microstructure (20-300 μm) with uniform pore distribution in all compositions. Materials were tested under compressive load in wet condition (using phosphate buffered saline at pH 7.4). The in vitro studies showed that all the scaffold compositions supported mesenchymal stem cell attachment, proliferation and differentiation as visible from SEM images, [3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide] (MTT) assay, alkaline phosphatase (ALP) assay and quantitative reverse transcription (qRT)-PCR.
Interpretation & conclusions: Scaffold composition 25CHT/HAP/PCL showed better biomechanical and osteoinductive properties as evident by mechanical test and alkaline phosphatase activity and osteoblast specific gene expression studies. This study suggests that this novel degradable 3D composite may have great potential to be used as scaffold in bone tissue engineering.
Stem Cell Facility, All India Institute of Medical Sciences, Ansari Nagar, New Delhi 110 029
|How to cite this article:|
Jain KG, Mohanty S, Ray AR, Malhotra R, Airan B. Culture & differentiation of mesenchymal stem cell into osteoblast on degradable biomedical composite scaffold: In vitro study.Indian J Med Res 2015;142:747-758
|How to cite this URL:|
Jain KG, Mohanty S, Ray AR, Malhotra R, Airan B. Culture & differentiation of mesenchymal stem cell into osteoblast on degradable biomedical composite scaffold: In vitro study. Indian J Med Res [serial online] 2015 [cited 2020 Nov 24 ];142:747-758
Available from: https://www.ijmr.org.in/article.asp?issn=0971-5916;year=2015;volume=142;issue=6;spage=747;epage=758;aulast=Jain;type=0