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CLINICAL IMAGE
Year : 2020  |  Volume : 152  |  Issue : 7  |  Page : 112-113

Comprehensive immune profiling in oral cancers


Department of Laboratory Haematology & Molecular Genetics, Tata Medical Center, Kolkata 700 160, West Bengal, India

Date of Submission19-Nov-2019
Date of Web Publication25-May-2021

Correspondence Address:
Neeraj Arora
Department of Laboratory Haematology & Molecular Genetics, Tata Medical Center, Kolkata 700 160, West Bengal
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/ijmr.IJMR_2182_19

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How to cite this article:
Karmakar T, Arora N. Comprehensive immune profiling in oral cancers. Indian J Med Res 2020;152, Suppl S1:112-3

How to cite this URL:
Karmakar T, Arora N. Comprehensive immune profiling in oral cancers. Indian J Med Res [serial online] 2020 [cited 2021 Jul 29];152, Suppl S1:112-3. Available from: https://www.ijmr.org.in/text.asp?2020/152/7/112/316765

Informed consent from patients with oral gingivobuccal squamous cell carcinoma procured for sample collection.


This work was performed at the Laboratory Haematology & Histopathology department, Tata Medical Center, Kolkata, India, from May 2017 to October 2019. Recent advancement in the immunotherapy include characterization of immune infiltrates (such as T cells, Tregs, B cells, NK cells, macrophages and dendritic cells) in tumour microenvironment (TME). In this study, immune cell subsets and checkpoint markers in the TME of oral-gingivobuccal squamous cell carcinoma have been analyzed by flow cytometry immunophenotyping (FCI) as well as immunohistochemistry (IHC). The uniqueness of this study is to identify, quantify and correlate immune cells (CD3, CD4 and CD8); inducible costimulatory molecule (CD278); cytolytic (granzyme) and checkpoint (TIM-3 and PD-1) molecules with the clinicopathological status of the disease and genomics using the same set of tumour samples. The novelty of this study is biomarker identification by FCI and detection of immune prognostication scores by IHC to address novel immunotherapeutic targets. The IHC images in [Figure 1]A, [Figure 1]B, [Figure 1]C, [Figure 1]D depict a representative case of oral gingivobuccal tumour with increased lymphoid infiltrate. These cells show high programmed death ligand-1 (PDL1) expression [Figure 1]E. The FCI image [Figure 1]G and [Figure 1]H shows increased CD3+ lymphocytes with expression of T cell immunoglobulin and mucin domain-containing-3 (TIM-3), PD-1 and CD278. Multiplex image [Figure 1]F also shows high expression of CD3, CD68, CD15 and granzyme. Evaluating TME will enhance our knowledge of oral cancer in management of patients for a better disease-free survival.
>Figure 1: Immunohistochemistry and flowcytometry images of oral cancer. (A) Irregular growth in lower Gingivobuccal region. (B) Excised tumour from oral cancer patient. (C) Haematoxylin and eosin stain (×40) showing nucleoli islands, each with moderate to abundant cytoplasm, and hyperchromatic nuclei. (D) Scattered brown DAB staining indicates CD3+ lymphocytes (×20). (E) Brown PDL1+ (×20) expressing cells with marginal infiltration. (F) Bright field view (×40) composite CD3, CD15, arginase, granzyme, CD68, nuclei in green, yellow, orange, magenta, red, blue, respectively. (G) TIM3+ PD1+ co-expression in blue monocytes and TIM3- PD1- expressed by green lymphocytes. (H) CD278+ in green lymphocytes & TIM3+CD278+ coexpression in the monocytic population.

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Financial support & sponsorship: Authors acknowledge the department of Biotechnology, India, for providing financial support [SyMeC: GIFT study (EC/GOVT/23/17): PI Dr Geetashree Mukherjee].

Conflicts of Interest: None.


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