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STUDENT IJMR
Year : 2020  |  Volume : 152  |  Issue : 3  |  Page : 312-315

Study of fine-needle aspiration microbiology versus wound swab for bacterial isolation in diabetic foot infections


1 Jawaharlal Institute of Postgraduate Medical Education & Research, Puducherry, India
2 Department of Microbiology, Jawaharlal Institute of Postgraduate Medical Education & Research, Puducherry, India
3 Department of Dermatology, Jawaharlal Institute of Postgraduate Medical Education & Research, Puducherry, India
4 Department of Surgery, Jawaharlal Institute of Postgraduate Medical Education & Research, Puducherry, India

Date of Submission21-Jun-2018
Date of Web Publication17-Oct-2020

Correspondence Address:
Dr Raj Kumar Nagarajan
Department of Surgery, Jawaharlal Institute of Postgraduate Medical Education & Research, Puducherry 605 007
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/ijmr.IJMR_1151_18

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   Abstract 

Background & objectives: Proper identification of the infection causing microbe in diabetic foot infections (DFIs) is essential for starting appropriate treatment. The objectives of this study were to compare fine-needle aspiration microbiology (FNAM) with wound swab as methods of sample collection in isolating microorganisms causing DFIs and also to compare the microbiological profile and sensitivity pattern of the infecting organisms.
Methods: This study was conducted targeting all consecutive patients with DFIs with perfusion, extent, depth, infection and sensation (PEDIS) grade 2, 3, and 4 infections admitted in the department of Surgery of a tertiary care hospital in south India during July to August 2017. A superficial wound swab and an FNAM were collected from all the patients. These swabs are analyzed using standard microbiological techniques.
Results: Eighty patients with DFI were included. Bacterial culture using FNAM samples yielded growth in 58.75 per cent samples, whereas wound swab samples yielded growth in 93.8 per cent cultures done. Measure of agreement between the two techniques using Kappa statistics was 0.069 (P=0.28).
Interpretation & conclusions: In diabetic wound infections, wound swabs were sufficient to identify organisms in all grades of infection. However, in deeper infections (grade 3 and 4), FNAM would be a reliable investigation than wound swab.

Keywords: Culture - diabetic foot infections - fine-needle aspiration microbiology - microorganism - wound swab


How to cite this article:
Abdulbasith K M, Bhaskar MM, Munisamy M, Nagarajan RK. Study of fine-needle aspiration microbiology versus wound swab for bacterial isolation in diabetic foot infections. Indian J Med Res 2020;152:312-5

How to cite this URL:
Abdulbasith K M, Bhaskar MM, Munisamy M, Nagarajan RK. Study of fine-needle aspiration microbiology versus wound swab for bacterial isolation in diabetic foot infections. Indian J Med Res [serial online] 2020 [cited 2020 Nov 30];152:312-5. Available from: https://www.ijmr.org.in/text.asp?2020/152/3/312/298466

Diabetes mellitus (DM) is a global problem and about 10-25 per cent diabetic patients develop ulcers[1]. According to the WHO Global Reports on Diabetes[2], “diabetic foot infections (DFIs) are an important cause of lower limb amputation which have significant impact on quality of life and can also incur catastrophic personal health expenditures”. Proper identification of the infection causing microbe is thus essential for starting appropriate treatment, which is required for proper wound healing[3],[4]. The method used for the collection of sample influences the quality of data on microbiological culture[5],[6]. Most commonly used method for sample collection is superficial wound swab for its ease and noninvasiveness[7], although unreliable, since wound swabs may also be contaminated by commensal organisms[4],[8]. Many studies have suggested deep tissue biopsy as the gold standard[4],[8],[9] but may not be always advisable due to concerns of spreading infection, ischaemia, or damaging adjacent structures. Fine-needle aspiration microbiology (FNAM) is less invasive than deep tissue biopsy and more sensitive than wound swab in predicting causative organisms[10],[11]. Hence, this study was performed to compare wound swab and FNAM methods for sample collection in the isolation of bacteria causing DFIs.


   Material & Methods Top


The present study was conducted among consecutive DFI patients admitted in the department of Surgery, Jawaharlal Institute of Postgraduate Medical Education & Research (JIPMER), a tertiary care centre in Puducherry, India, from July 1 to August 31, 2017. The study protocol was approved by the Institutional Ethics Committee and written informed consent was obtained from all participants.

Severity of the DFI was assessed by perfusion, extent, depth, infection and sensation (PEDIS) grading of International Working Group of the Diabetic Foot[12]. Patients with any two of the following signs such as local swelling or induration, erythema >0.5-2 cm around the ulcer, local tenderness or pain, local warmth or purulent secretion were graded as PEDIS grade 2. Patients with erythema >2 cm along with any one of the signs of grade 2 infections or infection involving structures deeper than skin and subcutaneous structures such as abscess, osteomyelitis, septic arthritis or fasciitis were graded as PEDIS grade 3. Any foot infection with signs of systemic inflammatory response syndrome (SIRS) was graded PEDIS 4.

Patients with a history of antibiotic intake during the previous four weeks, those with DFIs associated with dry gangrene and patients not willing to give consent were excluded from the study. At first, superficial wound swab was taken using Levine technique[13]. For FNAM, the surrounding non-ulcerated inflamed area within 2 cm of the wound was first cleaned with chlorhexidine gluconate and allowed to dry for 60 seconds. Fluid was aspirated from the suspected area using a 5 ml syringe and a 21G needle. Aspiration was done by introducing needle in the adjacent inflamed area within 2 cm of the wound and by briskly withdrawing the plunger multiple times. The content of the aspirate was transferred to a sterile wound swab. These swabs were sent to clinical microbiology laboratory for microscopy and culture and sensitivity using standard microbiological techniques. No local anesthetic agents was used for FNAM as some of these are shown to have anti-microbial property[14],[15].

Statistical analysis: The data analysis was performed using Statistical Package for the Social Sciences version 20 (IBM SPSS, Chicago, IL, USA). Age and sex were expressed as frequency and percentage. Comparison of these variables between the age group and sex was carried out by Chi-square test. The microbiologic profile and sensitivity pattern identified from FNAM and wound swab were summarized as frequency, percentage and 95 per cent confidence interval. Microorganisms isolated using wound swab and FNAM were compared using percentage agreement and Kappa statistics.


   Results & Discussion Top


A total of 80 patients with DFIs were included in the study. Of these 80, 72.5 per cent (n=58) were males. The mean age of the study population was 56±12.34 (27 to 80) yr. The study showed positive isolates by wound swab in 75 patients (93.8%) and FNAM-positive cultures in 47 patients (58.75%). Various organisms isolated are summarized in [Table 1]. This was in concordance with a study done by Gjødsbøl et al[16], who concluded that it was sufficient to use swab specimens to identify the bacterial species present in the chronic wounds. Demetriou et al[17] showed that swab cultures were highly sensitive but less specific and had good negative predictive value in diabetic patients.
Table 1: Isolates identified by fine-needle aspiration microbiology (FNAM) and wound swab samples

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In our study, the most common organism isolated was Escherichia coli by both FNAM and wound swab. The other common organisms isolated were Acinetobacter, Klebsiella, Pseudomonas, Enterobacter and Staphylococcus. FNAM showed more positive growth in grade 3 and 4 DFIs than grade 2 DFIs as depicted in [Table 2]. However, this did not attain significance owing to the small sample size of the study. The diagnostic accuracy of FNAM could not be established due to lack of gold standard (tissue culture) in our study. On comparing the organisms detected between FNAM and wound swab samples there was concordance in 32 (40%) cases with every organism isolated whereas in 37 (46.25%) cases there was no concordance in the organisms isolated [Table 3]. Absence of concordance may be because wound swab sampled superficial organisms/colonizers whereas FNAM could isolate organism in the deeper part of the wound. So FNAM could be a reliable investigation to isolate a true pathogen for higher PEDIS grade wounds.
Table 2: Correlation of fine.needle aspiration microbiology (FNAM) and wound swab yield to the grade of diabetic foot infection (DFI)

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Table 3: Concordance of organisms isolated by fine.needle aspiration microbiology and wound swab

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The major limitations of this study were small sample size and the lack of anaerobic culture. To conclude, our study showed that in diabetic wound infections, wound swabs were sufficient to identify organisms in all grades of infection. However, in deeper infections (grade 3 and 4), FNAM would be a better investigation than wound swab.

Financial support & sponsorship: The first author (AKMB) acknowledges the Indian Council of Medical Research, New Delhi, for providing Short Term Studentship (ICMR-STS No. 2017-02597).

Conflicts of Interest: None.



 
   References Top

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Singh N, Armstrong DG, Lipsky BA. Preventing foot ulcers in patients with diabetes. JAMA 2005 ; 293 : 217-28.  Back to cited text no. 1
    
2.
Roglic G, World Health Organization, editors. Global report on diabetes. Geneva, Switzerland: World Health Organization; 2016. p. 86.  Back to cited text no. 2
    
3.
Wheat LJ, Allen SD, Henry M, Kernek CB, Siders JA, Kuebler T, et al. Diabetic foot infections. Bacteriologic analysis. Arch Intern Med 1986 ; 146 : 1935-40.  Back to cited text no. 3
    
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Bozkurt F, Gülsün S, Tekin R, Hosoglu S, Acemoglu H. Comparison of microbiological results of deep tissue biopsy and superficial swab in diabetic foot infections. J Microbiol Infect Dis 2012 ; 2 : 122-7.  Back to cited text no. 4
    
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Lipsky BA. Medical treatment of diabetic foot infections. Clin Infect Dis 2004 ; 39 (Suppl 2) : S104-14.  Back to cited text no. 5
    
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Williams DT, Hilton JR, Harding KG. Diagnosing foot infection in diabetes. Clin Infect Dis 2004 ; 39 (Suppl 2) : S83-6.  Back to cited text no. 6
    
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Huang Y, Cao Y, Zou M, Luo X, Jiang Y, Xue Y, et al. A comparison of tissue versus swab culturing of infected diabetic foot wounds. Int J Endocrinol 2016 ; 2016 : 8198714.  Back to cited text no. 7
    
8.
Daivasikamani DDP. Comparing swab culture tissue culture to identify the infecting organism in diabetic foot ulcers. Sch Bull 2015 ; 5 : 11-5.  Back to cited text no. 8
    
9.
Bhabha Y, Tinley P, Davoren P, Derrington P. A pilot study comparing superficial wound swab deep tissue biopsy and fine needle aspiration biopsy in identifying infecting organisms in foot ulcers due to diabetes. J Foot Ankle Res 2011 ; 4 (Suppl 1) : P4.  Back to cited text no. 9
    
10.
Parikh AR, Hamilton S, Sivarajan V, Withey S, Butler PE. Diagnostic fine-needle aspiration in postoperative wound infections is more accurate at predicting causative organisms than wound swabs. Ann R Coll Surg Engl 2007 ; 89 : 166-7.  Back to cited text no. 10
    
11.
Sudharsanan S, Gs S, Sureshkumar S, Vijayakumar C, Sujatha S, Kate V. Does fine needle aspiration microbiology offer any benefit over wound swab in detecting the causative organisms in surgical site infections? Wounds 2017 ; 29 : 255-61.  Back to cited text no. 11
    
12.
Schaper NC. Diabetic foot ulcer classification system for research purposes: A progress report on criteria for including patients in research studies. Diabetes Metab Res Rev 2004 ; 20 (Suppl 1) : S90-5.  Back to cited text no. 12
    
13.
Levine NS, Lindberg RB, Mason AD Jr, Pruitt BA Jr. The quantitative swab culture and smear: A quick, simple method for determining the number of viable aerobic bacteria on open wounds. J Trauma 1976; 16 : 89-94.  Back to cited text no. 13
    
14.
Johnson SM, Saint John BE, Dine AP. Local anesthetics as antimicrobial agents: A review. Surg Infect (Larchmt) 2008 ; 9 : 205-13.  Back to cited text no. 14
    
15.
Schmidt RM, Rosenkranz HS. Antimicrobial activity of local anesthetics: Lidocaine and procaine. J Infect Dis 1970 ; 121 : 597-607.  Back to cited text no. 15
    
16.
Gjødsbøl K, Skindersoe ME, Christensen JJ, Karlsmark T, Jørgensen B, Jensen AM, et al. No need for biopsies: Comparison of three sample techniques for wound microbiota determination. Int Wound J 2012 ; 9 : 295-302.  Back to cited text no. 16
    
17.
Demetriou M, Papanas N, Panopoulou M, Papatheodorou K, Bounovas A, Maltezos E. Tissue and swab culture in diabetic foot infections: Neuropathic versus neuroischemic ulcers. Int J Low Extrem Wounds 2013 ; 12 : 87-93.  Back to cited text no. 17
    



 
 
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  [Table 1], [Table 2], [Table 3]



 

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