|Year : 2020 | Volume
| Issue : 6 | Page : 525-527
Why there are no effective herbal antidotes against snake venom available in India?
Antony Gomes1, Sourav Ghosh1, Aparna Gomes2
1 Department of Physiology, Laboratory of Toxinology & Experimental Pharmacodynamics, Department of Physiology, University of Calcutta, Kolkata 700 073, West Bengal, India
2 Division of New Drug Development, CSIR-Indian Institute of Chemical Biology, Kolkata 700 032, West Bengal, India
|Date of Submission||10-May-2018|
|Date of Web Publication||21-Jul-2020|
Division of New Drug Development, CSIR-Indian Institute of Chemical Biology, Kolkata 700 032, West Bengal
Source of Support: None, Conflict of Interest: None
|How to cite this article:|
Gomes A, Ghosh S, Gomes A. Why there are no effective herbal antidotes against snake venom available in India?. Indian J Med Res 2020;151:525-7
|How to cite this URL:|
Gomes A, Ghosh S, Gomes A. Why there are no effective herbal antidotes against snake venom available in India?. Indian J Med Res [serial online] 2020 [cited 2021 Jan 20];151:525-7. Available from: https://www.ijmr.org.in/text.asp?2020/151/6/525/290303
Snakebite is one of the major causes of death and disability in the tropical countries . The antidote [anti-snake venom serum (ASVS)] developed in 1894 by Calmette  remains the only therapeutic intervention despite having several limitations ,,. Before the development of ASVS, herbal antidote existed in nature, mentioned in age-old folk-traditional literature (Ayurveda, Unani, etc.) whose scientific validation began in the early 19th century . An exhaustive research was done by Mhaskar and Caius  (1898-1928) at Haffkine Institute, Bombay (now Mumbai), India, on the herbs active against snake venom. They concluded that none of the herbs/herbal combination tested was found effective against snake venom. One of the major causes of the failure of Mhaskar and Caius was that the concept of dose-response relationship [LD50/MLD (median lethal dose)] was not established at that time. Pharmacological-toxicological methodologies were available from the early 19th century, and a new dimension of research venture was started with herbs-snake venom. Our group at Kolkata (1981-2018) has established the efficacy of the herbal extract/compound active against Cobra and Viper venoms in animal models ,,,,,,, but an antidote could not be developed. In the present communication, some of the major constraints are discussed and analyzed for the future young researchers working on snake venom.
The starting material of this kind of research was the authentic snake venom, which was often not available in countries such as India, Nepal, Pakistan and Bangladesh. The term authentic means that (i) the snakes shall be captured from different parts (east, west, south and north) of the country and should be identified by a zoologist (taxonomist); (ii) age, sex, length and health status of the snakes should be recorded and kept at temperature-controlled serpentarium. Further, the snake from wild catch and snakes from captive breed should be kept separated because of venom qualities; (iii) the venom should be collected once in a month by a trained personnel in a sterile container with a record of the yield/snake and lyophilized immediately; (iv) lyophilized venom should be stored in amber-coloured screw-capped sterilized glass vials, labelled properly and stored in a cool temperature (8-15°C); and (v) biological testing (LD50/MLD in mice through intravenous, subcutaneous, intramuscular route) and biochemical properties (protein content, gel banding pattern, etc.) should be recorded. There is a need to establish National Snake Venom Bank (NSVB) for the above activity. The NSVB shall provide certificate of authentic venom for the bona fide buyers (for research), institute and industry (for ASVS production). The young researchers should be careful about the starting material and should go for certified authentic snake venom. Several other countries also supply certified authentic snake (of their country origin) venom at a high cost that will not serve our purpose of research. Therefore, there is a need to develop the NSVB and such efforts are in progress by the Department of Biotechnology [Biotechnology Industry Research Assistance Council (BIRAC)], India. One study has reported that ASVS manufactured by Indian companies fail to provide sufficient protection in snakebite victims . The reason could be non-availability of certified authentic snake venom in India.
The research on herbal antidote against snake venom needs a multidisciplinary approach where botanist, pharmacologists-physiologists, toxicologists and chemist-phytochemists may be involved. However, there are many hurdles among which procurement of authentic herbal resources is the main, which is also a basic need for any herbal research programme .
The collection of authentic herbal portions/parts (whole plant, root, stem, leaf, tuber, flower, etc.) requires the following: (i) the herbs should be identified by a plant taxonomist and a herbarium prepared should be preserved for future reference, (ii) age of the herbs, region and season of collection need to be recorded, and (iii) the herb washed with water, dried in sunlight, powdered and preserved in dry container at room temperature (15-22°C).
In the laboratory experiments, it is desirable to use aqueous extract (per oral), except when there is a need to purify the herbal pure compound, solvent (ethanol, methanol, chloroform, etc.) extracts may be used. Phytochemical purification, done by phytochemists, requires long protocols and skilled hands. It happens that purified compounds may have low-yield and low-activity, as compared with the whole extract. On several occasions the toxicity of the pure compound played a negative role . Therefore, for all herbal extracts/pure compounds toxicity studies are essential before safe human trials.
Herbal extracts/herbal compounds alone were not found to be effective in snake venom neutralization studies. It was established that herbal extracts/compounds when combined with nanoparticles may enhance the action of herbs . Gold nanoparticle with herbs increased the action of the herbs against snake venom ,,,,. Nanoparticles conjugation may decrease the action of the herbs and may show toxicity. The use of biodegradable and biocompatible nanoparticles (calcium, polymer, albumin nanoparticle) may be an alternative to avoid the toxicity. Newer methodologies such as in silico studies using molecular docking and modelling gained importance in snake venom research .
At present, there are no research training centres in India although snake venom research started in 1860 at Calcutta Medical College, Kolkata . Later, in 1930-1940, snake venom research continued at the Pharmacology Department of the Calcutta School of Tropical Medicine . In 2010, a registered society was formed in Kolkata with the name 'Toxinological Society of India' to promote snake venom research. There is a need to establish a Snake Venom Research Institute in India to fulfil the lacunae.
The knowledge gap, lack of facilities and funds in snake venom research are the major constraints in herbal antidote development against snake venom. Snake venom herbal antidote may be possible in the near future, provided there are cooperative hands between scientists, policy-makers and industry.
Conflict of Interest: None.
| References|| |
Warrell DA. Guidelines for the management of snake-bites. New Delhi: World Health Organization Regional Office for South-East Asia; 2010.
Calmette A. [L'immunisation artificielle des animaux contre le venin des serpents, et la thérapeutique expérimentale des morsures venimeuses]. CR Soc Biol
Sutherland SK. Serum reactions. An analysis of commercial antivenoms and the possible role of anticomplementary activity in de-novo reactions to antivenoms and antitoxins. Med J Aust
Corrigan P, Russell FE, Wainschel J. Clinical reactions to antivenin. In: Rosenberg P, editor. Toxins: Animal, plant and microbial
. Pergamon: Oxford; 1978. p. 457-65.
Chippaux JP, Goyffon M. Antivenom serotherapy: Its applications, its limitations, its future. Bull Soc Pathol Exot
Knowles R. The mechanism and treatment of snake bite in India. Trans R Soc Trop Med Hyg
Mhaskar KS, Caius JF. Indian plant remedies in snake bite. Indian J Med Res
Alam MI, Auddy B, Gomes A. Isolation, purification and partial characterization of viper venom inhibiting factor from the root extract of the Indian medicinal plant sarsaparilla (Hemidesmus indicus
R. Br.). Toxicon
Alam MI, Gomes A. Adjuvant effects and antiserum action potentiation by a (herbal) compound 2-hydroxy-4-methoxy benzoic acid isolated from the root extract of the Indian medicinal plant 'sarsaparilla' (Hemidesmus indicus
R. Br.). Toxicon
Alam MI, Gomes A. Viper venom-induced inflammation and inhibition of free radical formation by pure compound (2-hydroxy-4-methoxy benzoic acid) isolated and purified from anantamul (Hemidesmus indicus
R. BR.) root extract. Toxicon
Alam MI, Gomes A. Snake venom neutralization by Indian medicinal plants (Vitex negundo
and Emblica officinalis
) root extracts. J Ethnopharmacol
Chatterjee I, Chakravarty AK, Gomes A. Daboia russellii
and Naja kaouthia
venom neutralization by lupeol acetate isolated from the root extract of Indian sarsaparilla Hemidesmus indicus
R. Br. J Ethnopharmacol
Gomes A, Saha A, Chatterjee I, Chakravarty AK. Viper and cobra venom neutralization by beta-sitosterol and stigmasterol isolated from the root extract of Pluchea indica
Gomes A, Das R, Sarkhel S, Mishra R, Mukherjee S, Bhattacharya S, et al
. Herbs and herbal constituents active against snake bite. Indian J Exp Biol
Phillips RE, Theakston RD, Warrell DA, Galigedara Y, Abeysekera DT, Dissanayaka P, et al
. Paralysis, rhabdomyolysis and haemolysis caused by bites of Russell's viper
(Vipera russelli pulchella
) in Sri Lanka: Failure of Indian (Haffkine) antivenom. Q J Med
Farnsworth NR. The role of ethnopharmacology in drug development. Ciba Found Symp
Hudson A, Lopez E, Almalki AJ, Roe AL, Calderon AI. A review of the toxicity of compounds found in herbal dietary supplements. Planta Med
Gomes A, Ghosh S, Sengupta J, Datta P, Gomes A. Herbonanoceuticals: A new step towards herbal therapeutics. Med Aromat Plants
Saha K, Gomes A. Russell's viper venom induced nephrotoxicity, myotoxicity, and hepatotoxicity-Neutralization with gold nanoparticle conjugated 2-hydroxy-4-methoxy benzoic acid in vivo
. Indian J Exp Biol
Ghosh S, Gomes A. Russell's viper (Daboia russelli russelli
) venom toxicity neutralizing efficacy of curcumin- gold nanoparticle (C-GNP) in experimental animal model. J Toxins
Gomes A, Sengupta J, Ghosh S, Gomes A. Application of gold nanoparticle conjugation with 2-hydroxy-4-methoxy benzoic acid (HMBA) from Hemidesmus indicus
root enhancing neutralization of snake (Viper
) venom activity. J Nanosci Nanotechnol
Saha K, Ghosh S, Ghosh S, Dasgupta SC, Gomes A, Gomes A. Neutralization of Naja kaouthia
venom induced toxicity and stress response by Vitex negundo
-gold nanoparticle (VN-GNP) in experimental animal models. J Toxins
Bandopadhyay P, Halder S, Sarkar M, Kumar Bhunia S, Dey S, Gomes A, et al
. Molecular modeling of NK-CT1, from Indian monocellate cobra (Naja kaouthia
) and its docking interaction with human DNA topoisomerase II alpha. Bioinformation
Fayrer J. Destruction of life in India by poisonous snakes. Nature
Chopra RN, Iswariah UV. An experimental investigation into the action of the Venom of the Indian Cobra
? Naia naia vel tripudians. Indian J Med Res