|Year : 2019 | Volume
| Issue : 1 | Page : 7-22
Corneal transplantation in the modern era
Rashmi Singh, Noopur Gupta, M Vanathi, Radhika Tandon
Cornea, Cataract & Refractive Services, Dr Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Sciences, New Delhi, India
|Date of Submission||25-Jan-2019|
|Date of Web Publication||30-Sep-2019|
Dr. Radhika Tandon
Cornea, Cataract & Refractive Services, Dr Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Sciences, New Delhi 110 029
Source of Support: None, Conflict of Interest: None
| Abstract|| |
Corneal blindness is one of the major causes of reversible blindness, which can be managed with transplantation of a healthy donor cornea. It is the most successful organ transplantation in the human body as cornea is devoid of vasculature, minimizing the risk of graft rejection. The first successful transplant was performed by Zirm, and since then, corneal transplantation has seen significant evolution. It has been possible because of the relentless efforts by researchers and the increase in knowledge about corneal anatomy, improvement in instruments and advancements in technology. Keratoplasty has come a long way since the initial surgeries wherein the whole cornea was replaced to the present day where only the selective diseased layer can be replaced. These newer procedures maintain structural integrity and avoid catastrophic complications associated with open globe surgery. Corneal transplantation procedures are broadly classified as full-thickness penetrating keratoplasty and partial lamellar corneal surgeries which include anterior lamellar keratoplasty [sperficial anterior lamellar keratoplasty (SALK), automated lamellar therapeutic keratoplasty (ALTK) and deep anterior lamellar keratoplasty (DALK)] and posterior lamellar keratoplasty [Descemet stripping automated endothelial keratoplasty (DSAEK) and Descemet membrane endothelial keratoplasty (DMEK)] broadly.
Keywords: Corneal blindness - corneal transplantation - eye banking - graft rejection -keratoplasty - visual acuity
|How to cite this article:|
Singh R, Gupta N, Vanathi M, Tandon R. Corneal transplantation in the modern era. Indian J Med Res 2019;150:7-22
| Introduction|| |
Corneal transplantation or keratoplasty is the most commonly performed and also the most successful allogenic transplant worldwide. Zirm performed the first corneal transplantation in 1905. Since then, corneal transplantation has evolved from the replacement of full-thickness cornea to the replacement of selective diseased layers of the cornea. This has been possible because of the improvement in understanding of corneal anatomy, advanced surgical techniques, instruments and microscopes. Organ transplantation is a complex process with multiple legal, ethical and cultural issues. The corneal tissue has several characteristics that make storage and transplantation easier and the eye bank plays an important role in the whole process of corneal retrieval, storage and transplantation.
| Cornea: Structure and Function|| |
The cornea is a transparent and avascular structure of the eye, which constitutes the anterior-most part of the eyeball. It consists of six different anatomical layers [Figure 1]. The anterior-most is epithelium consisting of squamous cells, wing cells and basal cells. The second layer is Bowman's membrane, which has regenerative properties. Stroma constitutes the major part of the cornea and contains keratocytes and collagen lamellae that are densely distributed in the anterior as compared to the posterior stroma. Dua's layer is almost 10-15 μm in thickness and remains strongly adhered to overlying stromal fibres. The Descemet membrane provides a base for endothelial cells that have a key role in maintaining corneal transparency.
|Figure 1: Diagrammatic representation of anatomical layers of the cornea (Drawn by the authors based on the theoretical knowledge of the corneal anatomy).|
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| Corneal Transplantation: Evolution and Types|| |
In 1813 Himly first mentioned the concept of corneal transplantation, but Von Hippel actually performed the first transplant in 1886 by replacing human diseased cornea with that of rabbit cornea. Fuchs did the first lamellar keratoplasty (LK), which was not accepted well because of poor outcomes in terms of visual quality. The first successful corneal transplant was performed by Zirm in 1905. Keratoplasty can be done for various purposes and is classified as therapeutic, tectonic and optical. Therapeutic keratoplasty is done to remove the infective portion of the cornea mainly in cases of recalcitrant or perforated infective keratitis. Tectonic keratoplasty provides support and maintains the integrity of the globe. Optical keratoplasty aims to restore vision and has seen various advancements with time that has led to refinement in post-operative visual quality and outcomes. In the 19th century, LK that involves removal of selective corneal layers was initially used to treat anterior corneal opacities. It was used to treat corneal scars, keratoconus but discontinued due to suboptimal visual gain, which could be attributed to irregular interface or residual opacities. It was also associated with a longer learning curve. This led to an increase in the popularity of full-thickness penetrating keratoplasty (PKP), which provided better visual quality in that era. As full-thickness PKP was associated with its own set of problems such as risk of immune rejection, weak graft-host junction, suture-related complications such as loose sutures, suture-related infiltrates, astigmatism and longer recovery time, re-introduction of LK helped in solving many of these problems of full-thickness keratoplasty. Moffatt et al have described the various eras of corneal transplantation as the seven epochs depicting the major developments and advancements. [Figure 2] shows the evolution of corneal transplantation from the 17th century to the present time.
|Figure 2: Flowchart showing the evolution of corneal transplantation. Source: Ref. 7.|
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| Types of Corneal Transplantation|| |
There are various anatomical and clinical parameters that need to be evaluated before planning the type of corneal transplantation. A stepwise approach may be utilized while deciding the type of keratoplasty to be used for optimal management of a patient requiring corneal transplantation [Figure 3]. Corneal transplantation can be classified on the basis of indication for which it is being done (therapeutic, tectonic and optical). Similarly, there are different techniques, which are used for replacing selective anterior or posterior diseased part of cornea with a normal donor cornea. This technique of selective replacement of diseased part has many advantages in terms of less intra-operative complications, maintaining globe integrity and less chances of graft rejection in post-operative period as lesser amount of tissue is transplanted as compared to full-thickness PKP. Corneal transplantation is thus broadly classified as full-thickness PKP, anterior and posterior LKs (PLK) [Figure 4] & [Figure 5].
|Figure 3: Flowchart depicting a stepwise approach while planning surgical management in a case of corneal opacity. SALK, superficial anterior lamellar keratoplasty; HALK, hemi-automated lamellar keratoplasty; ALTK, automated lamellar therapeutic keratoplasty; DALK, deep anterior lamellar keratoplasty, DSAEK, Descemet stripping automated endothelial keratoplasty, DMEK, Descemet membrane endothelial keratoplasty.|
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|Figure 4: Diagrammatic representation of the type of keratoplasty depending the on the level of corneal opacities along with endothelial function (Drawn by the authors based on the practical knowledge utilized for decision making in cases of corneal opacity). SALK, superficial anterior lamellar keratoplasty; ALTK, automated lamellar therapeutic keratoplasty; DALK, deep anterior lamellar keratoplasty.|
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|Figure 5: Diagrammatic representation of the type of keratoplasty to be chosen for cases of corneal decompensation with compromised endothelium with and without stromal scarring. (Drawn by the authors based on the practical knowledge utilized for decision making in cases of corneal decompensation).|
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Anterior lamellar keratoplasty (ALK)
The lost technique of ALK was brought back in 1948 by Paufique et al. Microkeratome was introduced in 1964 by Barraquer. He used it to treat high refractive errors by the technique of keratomileusis and keratophakia. Microkeratome was able to give a more regular cut and thus avoid the poor visual gain because of the irregular interface. Kaufman modified the technique and introduced epikeratophakia, which described the use of lamellar graft without the need to perform host corneal dissection. Anterior LK has come a long way from manual dissection to microkeratome assisted and now to femtosecond laser-assisted keratoplasty. There are a variety of techniques described for anterior LK depending upon the depth of corneal opacity.
Superficial anterior lamellar keratoplasty (SALK): It was first described by Kaufman et al in 2003. It is used to treat corneal opacities involving anterior 30-40 per cent of the cornea. They did a sutureless surgery and replaced the host with a 200 μm lamellar graft using fibrin glue. It is mainly indicated for superficial scars as a result of healed keratitis, trachoma, trauma, superficial corneal dystrophies or degenerations [Figure 6].
|Figure 6: Post-operative clinical photograph of sutureless superficial anterior lamellar keratoplasty (SALK) for healed keratitis with superficial opacity involving anterior 200 μm of cornea. The photograph demonstrates a well apposed clear lamellar graft.|
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Automated lamellar therapeutic keratoplasty (ALTK): It is used for the treatment of anterior to midstromal corneal opacities. In this technique, the microkeratome is used to dissect both the host as well as the donor. It thus gives a better apposition and interface regularity between the host and donor graft [Figure 7],[Figure 8],[Figure 9].
|Figure 7: Intra-operative pictures showing use of automated microkeratome for host dissection (A) and donor dissection (B) in case of automated lamellar therapeutic keratoplasty (ALTK).|
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|Figure 8: Post-operative clinical photographs of a patient operated using automated lamellar therapeutic keratoplasty for right eye nebulomacular opacity.|
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|Figure 9: Clinical pictures showing post-operative diffuse (left) and slit images (right) of automated lamellar therapeutic keratoplasty (with sutures). 10-0 nylon monofilament=0.020-0.029 mm thickness.|
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Deep anterior lamellar keratoplasty (DALK): It is performed in cases of deep anterior corneal opacities with a good endothelial function. Various indications are deep stromal scars of healed keratitis, keratoconus and stromal dystrophies. It is of two types: pre-descemetic and descemetic. Pre-descemetic is done where the cornea is very thin and a high risk of perforation is expected. It aims to remove the pathology while still leaving a little posterior stroma along with intact endothelium. Descemet DALK aims to remove the complete stroma while leaving behind only bare Descemet's membrane. There are various techniques of performing DALK including manual dissection. Archila and Melles et al have described techniques for a deeper dissection using air or viscoelastic. Anwar and Teichmann remarkably improved the technique of deep DALK by introducing big bubble technique. Deep stromal injection of air is used to completely bare the Descemet's membrane [Figure 10].
|Figure 10: Intra-operative sequential steps of manual deep anterior lamellar keratoplasty in case of advanced keratoconus. (A) Keratoconus with paracentral ectasia; (B) lamellar dissection with the aid of intra-operative optical coherence tomography; (C) donor preparation; (D) at the end of surgery intra-operative optical coherence tomography showing well-apposed donor graft to host bed. OCT, optical coherence tomography.|
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Posterior lamellar keratoplasty (PLK)
These procedures are used to replace the endothelium and treat the conditions in which only endothelium (posterior-most layer of the cornea) is diseased and the rest of the cornea is not affected, e.g., Fuchs endothelial corneal dystrophy (FECD), posterior polymorphous corneal dystrophy (PPCD), congenital hereditary endothelial dystrophy (CHED), iridocorneal endothelial (ICE) syndrome, viral endothelial dysfunction, aphakic bullous keratopathy (ABK) and pseudophakic bullous keratopathy (PBK). The initial surgeons attempted to replace the posterior lamella of the cornea by doing an anterior dissection,, but this never became popular, and in 1956, Tillet introduced a posterior lamellar approach. Even though the posterior approach was used, the visual outcome and graft survival was poor which was attributed to manual dissection and use of sutures. Melles et al used air as tamponade agent for graft attachment to the host cornea instead of sutures. In this technique, a posterior stromal button was retrieved from a host by lamellar dissection and replaced by donor tissue through a 9 mm limbal incision. PLK was introduced by Melles et al in which the donor was folded and inserted through a 5 mm incision. The same technique was introduced as deep lamellar endothelial keratoplasty (DLEK) in the USA by Terry and Ousley. Melles et al further modified their technique and introduced Descemet stripping endothelial keratoplasty (DSEK). They modified the concept of host stromal dissection and introduced Descemet's stripping or descemeterorrhexis. In this technique, the Descemet's membrane-endothelium complex was removed by stripping and replaced by a posterior lamellar button from the donor tissue. DSEK became Descemet stripping automated endothelial keratoplasty (DSAEK) when Gorovoy used an automated microkeratome for donor tissue dissection. It made the learning curve easier as well the interface smoother and thus led to better visual outcomes. Melles et al again presented a breakthrough surgery in 2006 when they replaced only the diseased endothelium with a healthy endothelium by the technique of Descemet membrane endothelial keratoplasty (DMEK). It involves stripping the Descemet's membrane from the donor tissue but is associated with superior visual outcomes. EK is a better option as compared to PKP in patients with endothelial dysfunction of the cornea, as it minimizes the catastrophic intraoperative complications such as expulsive haemorrhage and choroidal detachment associated with an open globe surgery. It helps in early visual recovery with no suture-related complications and astigmatism. It is nowadays being considered as more of a refractive surgery owing to the early and better visual outcome [Figure 11].
|Figure 11: Post-operative day 1 clinical picture of operated Descemet stripping automated endothelial keratoplasty (DSAEK) showing well-apposed lenticule to host cornea.|
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| Review of Literature: Changing Trends|| |
Changing trends: Shift from full thickness to lamellar keratoplasty
Darlington et al did a review of corneal transplantation over a period from 1980 to 2004 and found that more than 95 per cent of corneal tissues were used for PKP, and the major indications were PBK, keratoconus, FECD and failed grafts. Röck et al did a study to evaluate the evolution of surgical procedures. They found that the mean rate of corneal transplantations doubled from 71 per year in the first six-year period to 139 per year in the second six-year period. Even though the number of PKP remained the same, the number of DMEK increased significantly from 2008 to 2016. It thus shows a remarkable change from full-thickness keratoplasties towards LKs. Zhang et al evaluated the trend of keratoplasty from 2000 to 2012, and found a significant decrease in the number of PKP and an increase in DSAEK and DALK since the introduction of lamellar keratoplasty in 2006. FECD was managed by DSAEK in 83 per cent cases and PKP in 13 per cent in 2011-2012. Le et al evaluated the current surgical approaches at the University of Toronto and found that 61 per cent of keratoplasties were LK and 39 per cent underwent full-thickness PKP. Amongst the lamellar procedures, DSAEK accounted for most of the surgeries (68%) followed equally by DALK (16%) and DMEK (16%). Zare et al during their study period of six years found the most common surgical technique to be PKP (70.9%), followed by DALK (20.1%) and DSAEK (2.3%). However, over the study period, there was a significant decrease in the number of PKP and increase in LK. Park et al in their 10 yr review found that there was an increase in the total number of corneal transplants from 2005 to 2014. In the last decade of their study, PKP was found to decrease significantly (from 95 to 42%) and an increase in LK techniques (from 5 to 58%) was seen. DSAEK was the most common (50%) type of corneal transplantation performed in the United States in 2014. The volume of DMEK was found to be doubling every year since 2011. About 75 per cent of cases of corneal decompensation were managed by EK in 2014. EK was the treatment of choice for more than 90 per cent cases of FECD and for 60 per cent of PBK cases; while PKP was being done for 40 per cent of cases of PBK.
Changing trends: Indications of keratoplasty
Corneal blindness is a major health problem worldwide. The indications for corneal transplantation are different from one region to another. Bullous keratopathy has been seen to be the most common indication for transplant in developed countries whereas infective keratitis and scars are more common in developing countries. There is a changing trend being noted in developing countries as well. Corneal diseases contributing to blindness in this region have also shifted to post-surgical bullous keratopathy (46.2%) and corneal degenerations (23.1%). Park et al evaluated the international distribution of corneal grafts from the Eye Bank Association of America (EBAA) and reported that the numbers of grafts increased from less than 1000 in 2005 to 24,400 grafts in 2014. The most common indications of corneal transplantation were PBK, keratoconus, FECD and failed grafts. PBK was the most common indication (20%) for PKP in 2005, which decreased significantly and became the third most common indication (9%) in 2014. FECD (22%) was the most common indication for corneal transplantation followed by PBK (12%), keratoconus (10%) and repeat corneal graft (10%) in 2014. There has been an increase in the number of PKP being performed for congenital opacities such as Peter's anomaly, sclerocornea, CHED and corneal dermoid. The most common indications for LK in this study were corneal degeneration, peripheral ulcerative keratitis, keratoconus and non-specific anterior lamellar stromal scarring. Keratoconus has increased significantly as an indication for LK since 2008 and is responsible for one-third cases of LK being performed.
Röck et al evaluated the indications for corneal transplantation and the changing trends of corneal transplantation from 2005 to 2016. The waiting list of patients increased from 36 patients in 2005 to 246 in 2016. The most common indications were found to be FECD (45.5%) and keratoconus (14.2%). Other indications for corneal transplantation were bullous keratopathy (10.4%), trauma (4.3%) and corneal graft failure due to rejection (8.9%), followed by infective keratitis and ulcers resulting due to trophic disease or chemical and thermal burns (16.8%). In 2005, corneal transplantation was most commonly done for keratoconus (41.7%); however, in 2016, it went down to the third most common indication (6.5%) as FECD significantly rose as the most common indication for transplant since 2013 and accounted for 69.5 per cent of transplants. Zhang et al reported FEC to a more common indication for transplantation as compared to PBK. Zare et al conducted a study in Iran over a period of six years from January 2004 to December 2009. They found keratoconus to be the most common indication for transplantation (38.4%) followed by bullous keratopathy (11.7%), failed grafts (10.6%), infective keratitis (10.1%) and trachomatous keratopathy. FECD accounted for only 0.8 per cent of transplants being done in Iran during the study period.
According to Le et al, the most common indication for PKP was graft failure (30%) followed by infection (18%), and keratoconus (17%). DSAEK was most commonly done for FECD (40%) and bullous keratopathy (33%). Keratoconus (57%) and corneal scarring (35%) were found to be the most common indications for DALK. DMEK was done mostly in cases of FECD (82%).
| Post-Operative Outcomes in Various Types of Keratoplasty|| |
Endothelial keratoplasty (EK)
In DSAEK, only the posterior lamella is replaced as compared to full-thickness PKP that gives it the benefit of faster and early visual recovery. Suture-related problems such as infiltrates, astigmatism and risk of rejection are also reduced. DSAEK is associated with a hyperopic shift of the order of approximately 0.8-1.5 D, depending on the lenticule thickness being transplanted. Hence, the intraocular lens power needs to be refined according to this phenomenon while doing EK combined with cataract surgery. The hyperopic shift is hypothesized to be due to the lenticular creating a negative power at the posterior corneal surface.
Visual outcome: The average visual acuity after DSAEK is about 20/40 as described in various studies,,. In a large series of 5160 patients of FECD, Shah et al found a mean visual acuity of 20/31. Similar results were given by others, with a reported mean visual acuity of 20/38 and 20/35, respectively. As the surgical techniques and visual outcomes are improving, so are the expectations of the patients. Even though visual results are great post-DSAEK, some patients are not happy which could be because of the interface irregularities,. There are reports in which well-functioning EK grafts have been replaced because of poor visual quality. This has been corroborated by confocal microscopy of donor-recipient interface and wavefront aberrometry, which also prove the recipient stroma to be the cause for visual quality degradation.
Complications of Descemet stripping automated endothelial keratoplasty
Graft failure: The incidence of primary graft failure has been well-documented post-PKP and has been reported to be approximately 10 per cent, but there are very few studies for DSAEK to document the rate of primary graft failure and loss of endothelial cell count per year. While a decrease of 15 per cent in endothelial cell density (ECD) is seen post-PKP,, a decrease of 36 per cent in ECD post-DSAEK has been documented by Terry et al. Price and Price found similar results and a drop of 34 per cent in their report on 263 post-DSAEK eyes. Terry et al evaluated the effect of donor, recipient and operative factors on graft success in the Cornea Preservation Time Study and found that graft success was more in cases of FECD, cases without any intra-operative complications and if the donor did not have diabetes.
Graft dislocation: The most common complication encountered post-DSAEK is dislocation of the graft, which requires rebubbling in the immediate to early post-operative period. The rate of dislocation has been reported to be 1-82 per cent. Price and Price included FECD patients and found a low rate (6.5%) of dislocations. However, other authors, have reported a dislocation rate of 26 and 23 per cent, respectively.
Graft rejection: As lesser amount of corneal tissue is transplanted in DSAEK as compared to PKP, we expect a lower rejection rate in DSAEK. It has also been seen that DSAEK patients present with very subtle rejection signs. The rejection episodes are usually asymptomatic and detected incidentally on routine examination. Various authors have reported similar graft rejection rates. Jordan et al did a study on 598 patients and found a rejection rate of 9 per cent. Allan et al reported a rejection rate of 7.5 per cent in DLEK cases and a similar rate of 7.5 per cent was reported by Terry et al, in their study on 80 eyes.
Other complications: Pupillary block glaucoma is very commonly seen after DSAEK surgery in immediate post-operative period because of the residual air bubble. It can be avoided by doing an intra-operative inferior peripheral iridectomy. Graft infection, infiltrates in the interface, endophthalmitis can also be seen after DSAEK. In the late post-operative period, epithelial ingrowth in the interface might occur. The incidence of pupillary block glaucoma has been reported to be between 0.1 and 9.5 per cent. Besides peripheral iridectomy, another way to avoid pupillary block is to leave a freely mobile air bubble after surgery. Terry et al followed this method and found only one case of a pupillary block in about 850 cases of DSAEK, i.e. a rate of 0.1 per cent.
The epithelial ingrowth in the interface is thought to occur because of epithelium gaining access into the interface through the full-thickness venting incisions that were used to release the interface fluid,. Epithelial ingrowth has also been seen in the anterior chamber and over the donor if there are vitreous attachments to the wound. Graft infection or interface infection is usually difficult to manage and may require therapeutic PKP ultimately. Another vision-threatening complication that can be seen post-DSAEK or PKP is endophthalmitis. It is even worse if caused due to fungal organisms.
Deep anterior lamellar keratoplasty (DALK)
Visual outcomes: Noble et al found best corrected visual acuity (BCVA) of 6/6 in 24.7 per cent cases while 84.9 per cent had visual acuity better than 6/12. Amayem and Anwar in a study on keratoconus patients found a visual acuity better than 6/9 in 95.8 per cent cases at one year post-operative follow up. Anwar and Teichmann reported a BCVA better than 6/9 in 27 per cent cases and Coombes et al found it to be 64 per cent at one year follow up. Watson et al reported BCVA of 6/6 or better in 64 per cent patients of PKP and 32 per cent of DALK patients. Funnell et al corroborated the above results in their cohort study with 70 per cent of PKP patients achieving BCVA of 6/6 or better as compared to 22 per cent patients of DALK achieving a similar visual outcome. However, there are some studies, which do not follow the above pattern. Trimarchi et al found the mean visual acuity to be higher in patients undergoing DALK as compared to PKP.
Complications of DALK
Descemet membrane (DM) perforation: Noble et al noted an incidence of 13.8 per cent DM perforation in their study. Various authors reported a variable percentage of DM perforation. While Watson et al corroborated the results of the above study with a 15 per cent rate of DM perforation, other authors reported rates as low as four per cent and as high as 39.2 per cent.
DM perforation is most likely to occur over the apex of the cone, which is the thinnest point and can be avoided by practicing caution while dissecting over thin cornea as well manipulating the spatula in a way that it remains superficial over the thin areas of the cornea. It can also occur while removing the peripheral ledge from host cornea and even during passing sutures. Sugita and Kondo reported this complication in 39.2 per cent of their cases, but none required conversion to PKP.
Graft rejection: DALK is rarely seen to be associated with graft rejection with reported rates to be as low as 5-8 per cent. Graft rejection post-DALK was first reported in 1973. It could be sight-threatening just like graft rejection seen in PKP and is avoidable by intensive steroid therapy. There are various advantages of DALK over PKP as DALK is a closed globe surgery and thus intraocular complications such as suprachoroidal haemorrhage, retinal detachment are reduced. Further, steroids are needed for a short period as well as in a lower dose because of the low risk of graft rejection.
| Penetrating Keratoplasty (Pkp)|| |
There have been various studies to evaluate the visual outcomes and complications post-PKP. It is done for full thickness opacities such as healed keratitis, post-traumatic scars, corneal dystrophies with endothelial involvement as well as corneal decompensation with anterior stromal scarring [Figure 12].
|Figure 12: Clinical photographs (A) showing operated penetrating keratoplasty with failed graft; (B) post-operative day one picture with clear graft and well-apposed graft-host junction.|
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Visual outcome: Brahma et al did a study on 18 patients with keratoconus to evaluate the visual outcome after PKP. They found an improvement in visual acuity, contrast sensitivity and glare acuity post-PKP. Their results were corroborated by an improvement in Visual Function-14 scores. Rahman et al reported a BCVA of more than 6/12 in 48 per cent patients at five years of follow up. Similarly, Beckingsale et al found that about 50 per cent of patients achieved visual acuity better than 6/18.
Graft rejection: Rahman et al reported an incidence of 21 per cent of graft rejection episodes, of which 7.4 per cent eventually went into graft failure. Pramanik et al reported a graft failure rate of 6.3 per cent at 20 years, and a similar failure rate of seven per cent has been reported in a study with 326 eyes. Pramanik et al found early graft failure to be rare, Olson et al did not report a single case of early graft failure within 24 months while Paglen et al reported five cases, i.e. 1.5 per cent of early graft failure.
Other complications: PK, being an open globe procedure, is associated with catastrophic complications such as expulsive choroidal haemorrhage. The introduction of LK significantly reduces the incidence of this grave complication. PKP is associated with suture-related problems such as loose sutures, suture infiltrates as well as suture-associated astigmatism. Post-PKP astigmatism is one of the common causes of low-vision post-keratoplasty clear graft. There are various methods of reducing suture-related astigmatism. Topography assisted suture rotation, selective suture removal/replacement, placement of compression sutures can help reduce astigmatism in early post-operative period. In late post-operative period, ablative procedures or arcuate keratotomies can be used. Another important cause of loss of vision post-PKP is post-PKP glaucoma. The various parameters which can contribute to an increase in the incidence of glaucoma are the presence of pre-existing glaucoma, increased intra-operative manipulation, severe inflammation in the post-operative period.
| Eye Banking in the Modern Era|| |
Organ donation is a complicated process as it involves many social, ethical and legal issues. As the number and techniques of corneal transplantations are increasing, so is the need for donor corneas contributing to a demand-supply shortage, especially in developing countries. To keep up with the growing demand of donor corneas, the first eye bank was started in New York by Paton in 1944. To further promote eye banking and establish uniform standards and rules, ten such eye banks in America joined hands to form the Eye Bank Association of America (EBAA). They were able to transplant 41,300 corneas during 1994 and the major source of their tissues were hospitals. Another achievement that helped in the progress of eye banking was the development of corneal storage media.
The revolution reached India in 1945 when the first eye bank was developed in Madras (now Chennai). In 1993, as reported by the various eye banks of India, approximately, 6413 corneal grafts were done and it has come a long way since then. Legislation to control eye banking and eye donation was first passed in India in 1957.
Legal issues in eye banking
The Transplantation of Human Organs Act was passed in 1994. It was mainly to control the illegal trafficking and sale of kidneys, but it mentioned: 'eyes and ears can be harvested anywhere by a registered medical practitioner'. There were still some legal hurdles like the need for a registered medical practitioner and the need for blood bank and intensive care unit. The Act was amended in 2011 after feedback from experts from National Eye Bank (NEB) and Eye Bank Association of India (EBAI). It was published in the National Gazette as Transplantation of Human Organs and Tissues Rules 2014. As per this Act, cornea was considered as tissue and not an organ, the consent for the donation was extended from legal next of kin to other relatives such as grandchildren and henceforth, trained technicians were allowed to retrieve corneas.
Another provision that has greatly increased eye retrieval is the introduction of Hospital Cornea Retrieval Programme (HCRP). A study was done to evaluate the factors affecting eye donation in post-mortem cases. It was done with the help of trained counsellors using a set format of questions. It was found that only 55.4 per cent people were aware of eye donation, of whom only 44.3 per cent volunteered to donate. This acknowledged the importance of active counselling by trained counsellors in hospital mortuaries and wards and the Delhi centralized Hospital Cornea Retrieval Programme (HCRP) was initialized in association with SightLife. It can work effectively if there is good coordination between the medical officers, nurse, health personnel, technicians, forensic experts, and the legal system.
Role of eye bank
Eye bank plays a great role in cornea harvesting, processing, and record keeping. It takes care of the various steps during harvesting, first of all, defining the optimum time between death and recovery. It is preferably taken as less than 12 h as there are fewer chances of infection as well a better quality of tissue. The eye bank keeps a vigil on controlling quality during harvesting, transportation, processing and storage until it reaches the surgeon. It is important to ensure complete asepsis during retrieval of the cornea as any source of infection can lead to vision-threatening complications such as graft infection and endophthalmitis. Multiple cases of post-keratoplasty graft infection have been reported because of the organisms present in the storage media. The donor scleral rim should always be sent for microbiological evaluation, and the patient should initially be kept on early follow up to pick up signs of graft infection and early intervention.
| Recent Advancements|| |
Various advancements in techniques have evolved which help to improve the outcomes of keratoplasty, of which intra-operative optical coherence tomography (iOCT) and femtosecond laser are most important.
Intra-operative optical coherence tomography (iOCT)
The iOCT provides continuous feedback of intra-operative surgical manoeuvres. It is very useful in LK such as SALK, ALTK, DALK, DSEK and DMEK. The iOCT helps in the measurement of central corneal thickness (CCT) of the donor as well as host cornea both of which are important parameters for deciding the blade size to be used in microkeratome for dissection. Furthermore, in cases of manual preparation of donor tissue, it acts as an intra-operative guiding tool to minimize complications. In cases of SALK, ALTK, proper apposition can be done with the help of iOCT. In DALK procedure, the iOCT guides every step of the surgery starting from depth of trephination to graft-host apposition. During big bubble DALK, the air needle can be passed under iOCT guidance to know the adequate depth for achieving big bubble, thereby decreasing chances of intra-operative perforation. In manual DALK, it helps in identifying a thinner location so that extra precaution can be taken while manoeuvring in and around the same area. Any small perforation in DALK can be identified easily and further steps may be taken to avoid the case getting converted to full thickness PKP. In cases of DSAEK, it helps in identifying the right orientation of graft and also ensures adequate apposition of host and donor cornea at the end of surgery. Small peripheral detachments, fluid pockets, reverse orientation of graft, interface debris can be picked up easily on iOCT, which would otherwise be very difficult to pick in hazy oedematous corneas under microscopic view. Juthani et al have concluded the presence of transient interface fluid seen on iOCT to be associated with the textural interface opacity in the long-term follow up. Again, in DMEK, right orientation of scroll can be identified by its configuration under iOCT. There are three different orientations, which have been described in iOCT of which DMEK scroll with upside rolling is considered as right orientation [Figure 13],[Figure 14].
|Figure 13: Clinical photograph of a child with left eye congenital corneal opacity along with an intra-operative photograph showing a very well-defined posterior corneal defect confirming the diagnosis of Peter's anomaly.|
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|Figure 14: Intra-operative photograph showing the well-apposed Descemet membrane endothelial keratoplasty (DMEK) lenticule on intra-operative optical coherence tomography.|
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Femtosecond laser-assisted lamellar keratoplasty (FALK)
FALK has many advantages over manual keratoplasty. Lamellar, as well as full-thickness PKP, can be performed using femtosecond laser. The sharp and accurate cut of different shapes in recipient and donor cornea results in perfect alignment of tissues reducing post-operative problems such as epithelial healing and astigmatism. It leads to better incision geometry, accurate graft-host apposition, and better wound healing. If the host and donor cuts are in perfect alignment, there is less postoperative astigmatism. It avoids a mismatch between host and donor as can be seen sometimes in manual keratoplasty with the trephination being eccentric. FALK increases graft-host interface surface area and thereby increasing the strength of graft-host junction and thus, decreasing risk for graft dehiscence. It is associated with less endothelial cell loss at the margin of a graft. During LK, it helps in smooth dissection. While microkeratome-assisted lamellar keratoplasty helps in dissecting a pre-decided depth of tissue, FALK, when combined with intra-operative OCT, helps in a more accurate analysis of the depth of opacity and preparation of customized grafts.
Several methods have been developed to manage the gap between donor availability and its requirement for visual rehabilitation. One of the methods is the development of bioengineered corneas. These are designed to replace the full thickness of the diseased cornea or a part of the diseased cornea. These range from keratoprosthesis [Figure 15], which replaces the function of the cornea to the recent development of tissue-engineered hydrogels that help in the regeneration of host tissues. Furthermore, there are bioengineered lenticules that can be used to correct the refractive errors by implantation into the cornea. There have been important developments in this area in the last few years with clinical trials being conducted for the artificial cornea, development of natural corneal replacements and biosynthetic matrices for host tissue regeneration. Buznyk et al conducted a study to assess the safety and efficacy of bioengineered corneas grafted as an alternative to human donor corneas in high-risk patients. They reported that bioengineered RHCIII-MPC (recombinant human collagen type III and 2-methacryloyloxyethyl phosphorylcholine) implants could be better alternatives to donor tissues in repairing corneas with severe pathologies but a large sample study size along with a longer follow up is needed to confirm the efficacy of such bioengineered implants.
|Figure 15 Clinical picture showing operated keratoprosthesis (K-pro) with operated membranectomy for retroprosthetic membrane.|
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| Conclusion|| |
Over a century has passed since the first corneal transplantation surgery was performed in Europe in 1905, and since then, there has been no looking back. Customized component corneal replacement strategies to replace diseased layers and conserve healthy components and ever improving instrumentation and engineering marvels have helped reach greater heights of sight restoration with faster recovery, better quality of vision, more reliable sight restoration, minimization of complications and greater longevity and success rates. Pari passu improvements in eye banking techniques have greatly helped the surgical results. The future holds promise with further optimization of results and pushing of boundaries of care, widening the scope of services to reach remote areas and underserved populations with the help of ever expanding horizons of medical science and engineering to approach new pinnacles.
Financial support & sponsorship: None.
Conflicts of Interest: None.
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[Figure 1], [Figure 2], [Figure 3], [Figure 4], [Figure 5], [Figure 6], [Figure 7], [Figure 8], [Figure 9], [Figure 10], [Figure 11], [Figure 12], [Figure 13], [Figure 14], [Figure 15]