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ORIGINAL ARTICLE
Year : 2017  |  Volume : 146  |  Issue : 6  |  Page : 780-787

Cedrus deodara: In vitro antileishmanial efficacy & immumomodulatory activity


1 Division of Microbiology, Rajendra Memorial Research Institute of Medical Sciences, Patna, India
2 Balaji Utthan Sansthan, Kala-azar Research Center, Patna, India
3 Department of Pharmaceutical Technology, School of Natural Product Studies, Jadavpur University, Kolkata, India
4 Department of Transplant Immunology & Immunogenetics, Division of Cellular Immunology, All India Institute of Medical Sciences, New Delhi, India

Correspondence Address:
Dr. Chandreshwar Prasad Thakur
Balaji Utthan Sansthan, Kala azar Research Center, Uma Complex, Fraser Road, Patna 800 001, Bihar
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/ijmr.IJMR_959_16

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Background & objectives: The existing antileishmanial drugs for complete cure of visceral leishmaniasis (kala-azar) are limited. The available drugs are either toxic or less effective leading to disease relapse or conversion to post-kala-azar dermal leishmaniasis. Several herbal extracts have been shown to have antileishmanial activity, but a herbal drug may not always be safe. In the present study, the extract of Cedrus deodara leaves has been standardized and tested for immunomodulatory antileishmanial activities. Methods: The extracts of C. deodara leaves with different solvents such as benzene, chloroform, ethyl acetate and methanol were made by soxhlation process. Solvents were removed under reduced pressure and temperature using rotary evaporator. The antileishmanial bioassay test was performed with in vitro maintained parasites. Immunomodulatory activity of different extracts was tested by flow cytometry. Standardization of the effective fraction was performed with Linalool as a marker compound through reverse-phase high-performance liquid chromatography. Results: The extract with the use of benzene solvent showed strong antileishmanial activities within a dose 25-200 μg/ml culture with non-significant haemolytic activities and significant immunomodulant activities against the host cells. Linalool was found to be 1.29 per cent in the effective extract of C. deodara. Interpretation & conclusions: The antileishmanial activity of C. deodara, as assessed by bioassay testing on Leishmania donovani parasites and immunomodulatory effect of benzene extract of leaves on host cells indicated that it might be a potential new safe therapeutic target to cure the visceral leishmaniasis.


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