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Year : 2016  |  Volume : 144  |  Issue : 4  |  Page : 566-571

Comparison of haemoglobin estimates using direct & indirect cyanmethaemoglobin methods

1 Centre for Promotion of Nutrition Research & Training with Special Focus on North-East, Tribal & Inaccessible Population (Indian Council of Medical Research), New Delhi, India
2 Department of Food & Nutrition, Lady Irwin College, University of Delhi, New Delhi, India
3 Department of Environmental Studies, National Institute of Occupational Health, Ahmedabad, India
4 National Institute of Medical Statistics, New Delhi, India
5 Technical Division, National Accreditation Board for Testing & Calibration Laboratories, New Delhi, India

Correspondence Address:
Gurudayal Singh Toteja
Centre for Promotion of Nutrition Research & Training with Special Focus on North East, Tribal & Inaccessible Population (ICMR), ICMR Campus II, 3 Red Cross Road, New Delhi 110 002
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0971-5916.200882

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Background & objectives: Estimation of haemoglobin is the most widely used method to assess anaemia. Although direct cyanmethaemoglobin method is the recommended method for estimation of haemoglobin, but it may not be feasible under field conditions. Hence, the present study was undertaken to compare indirect cyanmethaemoglobin method against the conventional direct method for haemoglobin estimation. Methods: Haemoglobin levels were estimated for 888 adolescent girls aged 11-18 yr residing in an urban slum in Delhi by both direct and indirect cyanmethaemoglobin methods, and the results were compared. Results: The mean haemoglobin levels for 888 whole blood samples estimated by direct and indirect cyanmethaemoglobin method were 116.1 ± 12.7 and 110.5 ± 12.5 g/l, respectively, with a mean difference of 5.67 g/l (95% confidence interval: 5.45 to 5.90, P<0.001); which is equivalent to 0.567 g%. The prevalence of anaemia was reported as 59.6 and 78.2 per cent by direct and indirect methods, respectively. Sensitivity and specificity of indirect cyanmethaemoglobin method were 99.2 and 56.4 per cent, respectively. Using regression analysis, prediction equation was developed for indirect haemoglobin values. Interpretation & conclusions: The present findings revealed that indirect cyanmethaemoglobin method overestimated the prevalence of anaemia as compared to the direct method. However, if a correction factor is applied, indirect method could be successfully used for estimating true haemoglobin level. More studies should be undertaken to establish agreement and correction factor between direct and indirect cyanmethaemoglobin methods.

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