Indan Journal of Medical Research Indan Journal of Medical Research Indan Journal of Medical Research Indan Journal of Medical Research
  Home About us Editorial board Search Ahead of print Current issue Archives Submit article Instructions Subscribe Contacts Login  
  Home Print this page Email this page Small font sizeDefault font sizeIncrease font size Users Online: 4038       

   Table of Contents      
Year : 2015  |  Volume : 141  |  Issue : 2  |  Page : 239-241

Salmonella enterica serovar Weltevreden ST1500 associated foodborne outbreak in Pune, India

1 Division of Bacteriology, National Institute of Cholera & Enteric Diseases (NICED), P-33, CIT Road, Scheme XM, Beliaghata, Kolkata, West Bengal, India
2 Department of Microbiology, B. J. Medical College, Pune, Maharashtra, India

Date of Web Publication21-Apr-2015

Correspondence Address:
Shanta Dutta
Division of Bacteriology, National Institute of Cholera & Enteric Diseases (NICED), P-33, CIT Road, Scheme XM, Beliaghata, Kolkata, West Bengal
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0971-5916.155595

Rights and Permissions

How to cite this article:
Jain P, Nandy S, Bharadwaj R, Niyogi SK, Dutta S. Salmonella enterica serovar Weltevreden ST1500 associated foodborne outbreak in Pune, India. Indian J Med Res 2015;141:239-41

How to cite this URL:
Jain P, Nandy S, Bharadwaj R, Niyogi SK, Dutta S. Salmonella enterica serovar Weltevreden ST1500 associated foodborne outbreak in Pune, India. Indian J Med Res [serial online] 2015 [cited 2020 Oct 27];141:239-41. Available from:


Foodborne gastroenteritis outbreaks due to non-typhoidal  Salmonella More Details represent important public health problem globally [1] . In the last two decades, Salmonella enterica serovar Weltevreden (S. Weltevreden) has emerged as a dominant foodborne pathogen globally especially in South-East Asian countries, being increasingly isolated from vegetables, meat and seafoods [1],[2],[3],[4],[5],[6] . S. Weltevreden associated foodborne outbreaks of gastroenteritis have been reported from Scandinavian countries, Réunion Island (France) and India in the past [7],[8],[9],[10] . Although, the complete genome sequence of S. Weltevreden outbreak strain 2007-60-3289-1 isolated in Scandinavia was available in EMBL nucleotide sequence database [7] , the implicating agent of S. Weltevreden associated outbreaks in India have not been characterized earlier.

Here we report occurrence of S. Weltevreden associated foodborne gastroenteritis outbreak among student staying in a hostel at Pune, Maharashtra, India, and characterization of the isolates by antimicrobial susceptibility pattern, plasmid profiling and typing, pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST).

On January 19, 2010, 150 students aged between 20-30 yr had consumed food comprising milk, rice, sprouted pulses and curd from their hostel canteen for lunch in Pune, Maharashtra. Almost all of them developed acute watery diarrhoea within 12 h of having food, associated with fever, abdominal cramps, nausea and vomiting. Most of them were immediately admitted to the local private hospitals (Aditya Birla Group Hospital) for treatment. Stool samples (n=25) were collected from the admitted patients, processed by standard microbiological method and the isolated organism (n=14) were sent to B. J. Medical College, Pune, for identification. The patients were discharged following treatment with either ciprofloxacin metronidazole combination or ceftriaxone alone. Food samples were not available to identify the source of infection due to delay (>12 h) in carrying out the investigation. A total of nine isolates, provisionally identified as Salmonella spp. were sent to National Institute of Cholera and Enteric Diseases (NICED), Kolkata, for confirmation of identification and characterization.

At NICED, the Salmonella isolates were identified by standard biochemical tests, followed by serotyping using Salmonella specific poly- and monovalent O and H antisera (Denka Seiken Pvt. Ltd, Tokyo, Japan) and nomenclature was done following Kauffmann White Scheme [11] . The isolates were tested for their antimicrobial susceptibility using 17 antibiotic discs (BD BBL™, Marryland, USA); ampicillin, chloramphenicol, tetracycline, co-trimoxazole, nalidixic acid, ciprofloxacin, norfloxacin, ofloxacin, gentamicin, amikacin, streptomycin, cefotaxime, ceftazidime, ceftriaxone, aztreonam, azithromycin and erythromycin, by disc diffusion method and results were interpreted following CLSI (Clinical and Laboratory Standards Institute) guidelines [12] .  Escherichia More Details coli ATCC 25922 was used as control. Plasmid DNA was extracted by Kado and Liu method [13] , electrophoresed on 0.8 per cent agarose gel and stained with ethidium bromide to determine the plasmid profiles. E. coli V517 was used as plasmid molecular weight marker. The plasmid incompatibility type was determined by PCR using published primers followed by sequencing [14] .

DNA fingerprinting was performed by PFGE in CHEF-DRIII system (BioRad Laboratories, Hercules, CA, USA), following standard PulseNet one-day protocol ( and using Salmonella Braenderup H9812 as control. The PFGE profiles were analyzed using FP Quest TM software version 4.5 (Bio-Rad, CA, USA). The extent of homology was determined by Dice coefficient, and clustering was based on unweighted pair group method with arithmetic mean (UPGMA) [15] . The isolates with ≥ 90 per cent similarity threshold were grouped under one cluster.

Phylogenetic relatedness of the isolates was studied by MLST. The amplification of the seven housekeeping genes followed by sequencing was done as previously described [16] . Sequences obtained were submitted to the MLST database ( to assign sequence type (ST) of each isolate based on the set of allele types derived from all the seven loci.

Two local S. Weltevreden isolates from sporadic diarrhoea cases in Kolkata were also included in the study and characterized for comparison.

Out of nine outbreak isolates, eight were identified as S. Weltevreden. All the study isolates (Pune outbreak and Kolkata sporadic) were uniformly susceptible to the 17 antimicrobials tested. Pan susceptible S. Weltevreden isolates have earlier been reported from outbreaks in France (2007) and Mangalore (2008) [8],[9] . However, isolation of multi-drug resistant S. Weltevreden (resistant to ampicillin, streptomycin, co-trimoxazole, erythromycin and tetracycline) has also been reported from environmental samples in different countries [2],[3],[4],[5],[6] .

Single plasmid of 63kb size belonging to IncFIIS type was observed in all the study isolates. Earlier studies have documented presence of single or more plasmids ranging from <2 to 89.1 kb sizes [5] . The plasmids of type IncFIIS are considered as virulence plasmids in Salmonella[14], but its role in disease pathogenesis is yet to be established.

The Pune outbreak (A) and Kolkata sporadic (B) isolates formed distinct clusters in PFGE ([Figure 1]). All the outbreak isolates were found to be clonal (100%) and had 87 per cent similarity with the sporadic isolates. MLST database characterized all the study isolates as ST1500 [Clonal complex (CC) type 205] based on their sequence data. The allelic types of the seven genes were aroC: 130, dnaN: 97, hemD: 25, hisD: 125, purE: 422, sucA: 9, and thrA: 101. The current data have been entered in the MLST database and are available at the website ( S. Weltevreden human isolates from Sri Lanka, Australia, Indonesia, Zimbabwe, Philippines and Germany belonging to ST365 and that of an environmental isolate from UK belonging to ST1500 were available in the aforesaid MLST database. This report showed that the foodborne outbreak caused by Indian S. Weltevreden isolates belonged to ST1500 (CC205).
Figure 1. Pulsed field gel electrophoresis (PFGE) profiles of XbaI digest of DNA of S. Weltevreden Pune outbreak isolates, 2010 by cluster analysis and comparison with S. Weltevreden Kolkata sporadic isolates. Band comparison was performed using Dice coefficient with 1.50 per cent optimization (Opt) and 1.50 per cent position tolerance (Tol). H, minimum height; S, minimum surface; mo, months; M, male; F, female.

Click here to view

The use of molecular methods and antibiotic susceptibility profiles for characterization of Salmonella outbreak isolates in various geographical regions is useful for studying global epidemiology of the organism which might result in controlling further spread of the organism.

   Acknowledgment Top

The study was supported by the Indian Council of Medical Research (ICMR) intramural fund. ICMR-SRF to the first author (PJ) and technical assistance by NICED staff are acknowledged. Authors also thank the Curator of the MLST database (, which is currently supported by a grant from the Science Foundation of Ireland (05/FE1/B882) for enabling us to submit our data.

   References Top

World Health Organization Global Salm-Surv. Progress Report (2000-2005): Building capacity for laboratory-based foodborne disease surveillance and outbreak detection and response. WHO. 2006. Available from, accessed on July 7, 2012.  Back to cited text no. 1
Aarestrup FM, Lertworapreecha M, Evans MC, Bangtrakulnonth A, Chalermchaikit T, Hendriken RS, et al. Antimicrobial susceptibility and occurrence of resistance genes among Salmonella enterica serovar Weltevreden from different countries. J Antimicrob Chemother 2003; 52 : 715-8.   Back to cited text no. 2
Sirichote P, Bangtrakulnonth A, Tianmanee K, Unahalekhaka A, Oulai A, Chittaphithakchai P, et al. Serotypes and antimicrobial resistance of Salmonella enterica ssp. in central Thailand, 2001-2006. Southeast Asian J Trop Med Public Health 2010; 41 : 1405-15.  Back to cited text no. 3
Thong KL, Goh YL, Radu S, Noorzaleha S, Yasin R, Koh YT, et al. Genetic diversity of clinical and environmental strains of Salmonella enterica serotype Weltevreden isolated in Malaysia. J Clin Microbiol 2002; 40 : 2498-503.  Back to cited text no. 4
Ponce E, Khan AA, Cheng C, Summage-West C, Cerniglia CE. Prevalence and characterization of Salmonella enterica serovar Weltevreden from imported seafood. Food Microbiol 2008; 25 : 29-35.  Back to cited text no. 5
Deekshit VK, Kumar BK, Rai P, Srikumar S, Karunasagar I, Karunasagar I. Detection of class 1 integrons in Salmonella Weltevreden and silent antibiotic resistance genes in some seafood-associated nontyphoidal isolates of Salmonella in south-west coast of India. J Appl Microbiol 2012; 112 : 1113-22.  Back to cited text no. 6
Brankatschk K, Blom J, Goesmann A, Smits THM, Duffy B. Genome of an European fresh-vegetable food safety outbreak strain of Salmonella enterica subsp. enterica serovar Weltevreden. J Bacteriol 2011; 193 : 2066.   Back to cited text no. 7
D′Ortenzio E, Weill FX, Ragonneau S, Lebon JA, Renault P, Pierre Vl. First report of a Salmonella enterica serovar Weltevreden outbreak on Réunion Island, France, August 2007. Euro Surveill 2008; 13 : 7-9.  Back to cited text no. 8
Antony B, Dias M, Shetty AK, Rekha B. Food poisoning due to Salmonella enterica serotype Weltevreden in Mangalore. Indian J Med Microbiol 2009; 27 : 257-8.  Back to cited text no. 9
Chowdhury G, Sarkar A, Pazhani GP, Mukhopadhyay AK, Bhattacharya MK, Ramamurthy T. An outbreak of foodborne gastroenteritis caused by dual pathogens, Salmonella enterica Serovar Weltevreden and Vibrio fluvialis in Kolkata, India. Foodborne Pathog Dis 2013; 10 : 904-6.   Back to cited text no. 10
Grimont PAD, Weill FX. Antigenic formulae of the Salmonella serovars, 9 th ed. Paris: World Health Organization Collaborating Centre for Reference and Research on Salmonella, Institut Pasteur; 2007.  Back to cited text no. 11
Clinical and Laboratory Standards Institute (CLSI). Performance standards for antimicrobial susceptibility testing; 22 nd Informational Supplement. CLSI document M100-S22. Wayne, PA: CLSI; 2012.  Back to cited text no. 12
Kado CI, Liu ST. Rapid procedure for detection and isolation of large and small plasmids. J Bacteriol 1981; 145 : 1365-73.  Back to cited text no. 13
Villa L, García-Fernández A, Fortini D, Carattoli A. Replicon sequence typing of IncF plasmids carrying virulence and resistance determinants. J Antimicrob Chemother 2010; 65 : 2518-29.  Back to cited text no. 14
Tatavarthy A, Sanderson R, Peak K, Scilabro G, Davenhill P, Cannons A, et al. Molecular typing and resistance analysis of travel-associated Salmonella enterica serotype Typhi. J Clin Microbiol 2012; 50 : 2631-8.  Back to cited text no. 15
Noda T, Murakami K, Asai T, Etoh Y, Ishihara T, Kuroki T, et al. Multi-locus sequence typing of Salmonella enterica subsp. enterica serovar Enteritidis strains in Japan between 1973 and 2004. Acta Vet Scand 2011; 53 : 38.  Back to cited text no. 16


  [Figure 1]

This article has been cited by
1 Atypical Salmonella enterica Serovars in Murine and Human Macrophage Infection Models
Daniel Hurley,Maria Hoffmann,Tim Muruvanda,Marc W. Allard,Eric W. Brown,Marta Martins,Séamus Fanning,Andreas J. Bäumler
Infection and Immunity. 2020; 88(4)
[Pubmed] | [DOI]
2 Prevalence of MDR salmonellae with increasing frequency of S. Kentucky and S. Virchow serovars among hospitalized diarrheal cases in and around Delhi, India
Naresh Chand Sharma,Dhirendra Kumar,Anirban Sarkar,Goutam Chowdhury,Asish K Mukhopadhyay,Thandavarayan Ramamurthy
Japanese Journal of Infectious Diseases. 2019;
[Pubmed] | [DOI]
3 Salmonella in Retail Food and Wild Birds in Singapore—Prevalence, Antimicrobial Resistance, and Sequence Types
G. A. S. Aung,G. A. S. Chen,G. A. S. Chau,G. A. S. Yap,G. A. S. Lim,G. A. S. Humaidi,G. A. S. Chua,G. A. S. Yeo,G. A. S. Yap,G. A. S. Oh,G. A. S. Manogaran,G. A. S. Hapuarachchi,G. A. S. Maiwald,G. A. S. Tee,G. A. S. Barkham,G. A. S. Koh,G. A. S. Gutiérrez,G. A. S. Schlundt,G. A. S. Ng
International Journal of Environmental Research and Public Health. 2019; 16(21): 4235
[Pubmed] | [DOI]
4 Outbreak of S. Weltevreden linked to fermented cashew nut cheese in Victoria, BC
Nicolas Schmitt,Gloria Yu,Richard Greve,Lorraine McIntyre
Environmental Health Review. 2018; 61(3): 74
[Pubmed] | [DOI]
5 Application of the Subtractive Genomics and Molecular Docking Analysis for the Identification of Novel Putative Drug Targets against Salmonella enterica subsp. enterica serovar Poona
Tanvir Hossain,Mohammad Kamruzzaman,Talita Zahin Choudhury,Hamida Nooreen Mahmood,A. H. M. Nurun Nabi,Md. Ismail Hosen
BioMed Research International. 2017; 2017: 1
[Pubmed] | [DOI]


    Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
    Access Statistics
    Email Alert *
    Add to My List *
* Registration required (free)  

  In this article
    Article Figures

 Article Access Statistics
    PDF Downloaded258    
    Comments [Add]    
    Cited by others 5    

Recommend this journal