Indan Journal of Medical Research Indan Journal of Medical Research Indan Journal of Medical Research
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Year : 2012  |  Volume : 136  |  Issue : 6  |  Page : 971-978

Improvised microinjection technique for mosquito vectors

Department of Sericulture & Division of Biological Sciences, School of Natural Sciences, Bangalore University, Bangalore, India

Correspondence Address:
H P Puttaraju
Department of Sericulture & Division of Biological SciencesSchool of Natural Science, Bangalore University, Bangalore 560 056
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Source of Support: None, Conflict of Interest: None

PMID: 23391792

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Background & objectives: Bio-manipulation technique is of primary importance during the development of transgenic mosquitoes. The study describes the variable factors that influence the viability of medically important mosquito vectors during microinjection. Methods: Three mosquito vectors belonging to the genus Aedes, Anopheles and Culex were microinjected at different developmental stages of their life cycle viz., egg, larvae, pupae and adult. Results: The improvisations revealed an increased survivability of biomanipulated mosquitoes during the embryonic and adult microinjection. The study of injecting larvae and pupae resulted in poor survivability. Interpretation & conclusions: The microinjection protocol was successfully tested on three important mosquito vectors. The critical period after biomanipulation which contributes heavily for the survivability factor was evaluated. The results provide a common protocol for biomanipulation of three mosquito vectors with enhanced survivability.

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