Indan Journal of Medical Research Indan Journal of Medical Research Indan Journal of Medical Research Indan Journal of Medical Research
  Home About us Editorial board Search Ahead of print Current issue Archives Submit article Instructions Subscribe Contacts Login  
  Home Print this page Email this page Small font sizeDefault font sizeIncrease font size Users Online: 808       
ORIGINAL ARTICLE
Year : 2012  |  Volume : 136  |  Issue : 5  |  Page : 822-826

Antioxidant & anticancer activities of isatin (1H-indole-2,3-dione), isolated from the flowers of Couroupita guianensis Aubl


1 Department of Biotechnology, Mepco Schlenk Engineering College, Sivakasi, India
2 Department of Instrumentation, CSIR-Central Electro Chemical Research Institute, Karaikudi, India
3 Electroorganic Division, CSIR-Central Electro Chemical Research Institute, Karaikudi, India
4 Department of Zoology, Thiruvalluvar University, Vellore, India
5 Department of Microbiology, Ayya Nadar Janaki Ammal College, Sivakasi, India
6 Centre of Advanced Study in Marine Biology, Annamalai University, Parangipettai, India

Correspondence Address:
Mariappan Premanathan
Department of Biotechnology, Mepco Schlenk Engineering College, Mepco Nagar, Sivakasi 608 502, Tamil Nadu
India
Login to access the Email id

Source of Support: None, Conflict of Interest: None


PMID: 23287130

Rights and PermissionsRights and Permissions

Background & objectives: Derivatives of isatin are known to have cytotoxicity against human carcinoma cell lines. This compound therefore, has a potential to be used as a chemotherapeutic agent against cancer. This study was done to investigate the antioxidant and anticancer activities of isatin, extracted from flower of a folklore medicinal plant Couroupita guianensis against human promylocytic leukemia (HL60) cells. Methods: Active fractions demonstrating anticancer and antioxidant activities were isolated from the extracts of shade-dried flowers of C. guianensis by bioassay guided fractionation. The free radical scavenging activity was determined using lipid peroxidation assay. Cytotoxicity against human promylocytic leukemia HL60 cells was determined by MTT assay. Apoptotic activity was analyzed by DNA fragmentation and flowcytometry. Results: Isatin isolated from the active fraction showed antioxidant activity with the EC 50 value of 72.80 μg/ml. It also exhibited cytotoxicity against human promylocytic leukemia HL60 cells in dose-dependant manner with the CC 50 value of 2.94 μg/ml. The isatin-treated cells underwent apoptosis and DNA fragmentation. Apoptosis was confirmed by the FACS analysis using FITC-annexin V markers. Interpretation & conclusions: Isatin showed antioxidant activity and was cytotoxic to the HL60 cells due to induction of apoptosis. The isatin can be further evaluated to be used as a prophylactic agent to prevent the free radical-induced cancer and as a chemotherapeutic agent to kill the cancer cells.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed2764    
    Printed25    
    Emailed0    
    PDF Downloaded475    
    Comments [Add]    

Recommend this journal