| ORIGINAL ARTICLE |
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| Year : 2011 | Volume
: 133
| Issue : 4 | Page : 421-425 |
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Mycobacterial heparin-binding haemagglutinin adhesion-induced interferon & antibody for detection of tuberculosis
Zhaogang Sun, Lihui Nie, Xuxia Zhang, Yan Li, Chuanyou Li
Department of Bacteriology & Immunology, Beijing Tuberculosis & Thoracic Tumor Research Institute, Beijing, China
Correspondence Address:
Chuanyou Li
Department of Bacteriology & Immunology, Beijing Tuberculosis & Thoracic Tumor Research Institute, Beijing, 101149
China
 Source of Support: None, Conflict of Interest: None  | Check |
PMID: 21537096 
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Background & objectives : Mycobacterial heparin-binding haemagglutinin adhesin (HBHA) plays an important role in humoral and cellular immune response and is a potential diagnostic tool for tuberculosis (TB) serodiagnosis. This study was carried out to assess the usefulness of HBHA in TB clinics for differential diagnosis of pulmonary and extra-pulmonary TB (PTB, EPTB).
Methods: In this study, 165 outpatients and 133 healthy volunteers were included to investigate the role of HBHA in TB diagnosis including the serodiagnostic tests and the interferon-γ release assays (IGRAs). The healthy volunteers were all without BCG vaccination including 73 subjects with purified protein derivative (PPD) (-) and 60 ones with PPD (+) (that is P-B- and P+B-). Of all the 165 outpatients 77 were PTB and 88 were EPTB. HBHA protein was used for serodiagnostic tests and IGRAs in peripheral blood mononuclear cells.
Results: HBHA-specific antibody levels in the serum of healthy subjects were significantly different from the patients with PTB or EPTB (P<0.05). HBHA specific antibody levels in PTB patients could differentiate from EPTB with limited sensitivity (77.08%; 95%CI, 62.69 to 87.97%) and specificity (87.50%; 95%CI, 74.75 to 95.27%). IFN-γ levels in the healthy (P+B- and P-B-) groups were significantly different (P<0.01) with a detection sensitivity of 84.8% (95%CI, 68.54 to 93.02%) and specificity of 80.7% (95%CI, 65.22 to 92.62%). The PTB and EPTB subjects showed no difference in IFN-γ production.
Interpretation & conclusions : HBHA serodiagnostic test with IGRAs had the limited potential for use as auxiliary tools for the differential diagnosis of PTB and EPTB, since both methods showed low sensitivity and specificity. |
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