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  Indian J Med Microbiol
 

Figure 1: Workflow demonstrating systematic investigations leading to identification of desmin (DES) as an epigenetically regulated gene in gall bladder cancer. MS-PCR, methylation-specific polymerase chain reaction; qRT-PCR, quantitative reverse transcription-polymerase chain reaction; IHC, immunohistochemistry; iTRAQ, isobaric tags for relative and absolute quantitation; LC-MS/MS, liquid chromatography-tandem mass spectrometry; DMRs, differentially methylated regions.

Figure 1: Workflow demonstrating systematic investigations leading to identification of desmin (<i>DES</i>) as an epigenetically regulated gene in gall bladder cancer. MS-PCR, methylation-specific polymerase chain reaction; qRT-PCR, quantitative reverse transcription-polymerase chain reaction; IHC, immunohistochemistry; iTRAQ, isobaric tags for relative and absolute quantitation; LC-MS/MS, liquid chromatography-tandem mass spectrometry; DMRs, differentially methylated regions.