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  Indian J Med Microbiol
 

Figure 2: PCR products of duplex MAMA-PCR assays (A) MAMA gyrA 83. M1: 100 bp DNA ladder; lane 1: gyrA PCR; lane 2: negative control; lane 3: gyrA 83 without mutation; lane 4: gyrA 83 with mutation. (B) MAMA gyrA 87. M2:100 bp DNA ladder; lane 1: gyrA PCR; lane 2: negative control; lane 3: gyrA 87 without mutation; lane 4: gyrA 87 with mutation. (C) MAMA parC 80. M1:100 bp DNA ladder; lane 1: parC PCR; lane 2: negative control; lane 3: parC 80 without a mutation; lane 4: parC 80 with a mutation. (D) MAMA parC 84. M2:100 bp DNA ladder; lane 1: parC PCR; lane 2: negative control; lane 3: parC 84 without mutation; lane 4: parC 84 without mutation. MAMA-PCR, mismatch amplification mutation assays-polymerase chain reaction.

Figure 2: PCR products of duplex MAMA-PCR assays (<b>A</b>) MAMA <i>gyrA</i> 83. M1: 100 bp DNA ladder; lane 1: <i>gyrA</i> PCR; lane 2: negative control; lane 3: <i>gyrA</i> 83 without mutation; lane 4: <i>gyrA</i> 83 with mutation. (<b>B</b>) MAMA <i>gyrA</i> 87. M2:100 bp DNA ladder; lane 1: <i>gyrA</i> PCR; lane 2: negative control; lane 3: <i>gyrA</i> 87 without mutation; lane 4: <i>gyrA</i> 87 with mutation. (<b>C</b>) MAMA <i>parC</i> 80. M1:100 bp DNA ladder; lane 1: <i>parC</i> PCR; lane 2: negative control; lane 3: <i>parC</i> 80 without a mutation; lane 4: <i>parC</i> 80 with a mutation. (<b>D</b>) MAMA <i>parC</i> 84. M2:100 bp DNA ladder; lane 1: <i>parC</i> PCR; lane 2: negative control; lane 3: <i>parC</i> 84 without mutation; lane 4: <i>parC</i> 84 without mutation. MAMA-PCR, mismatch amplification mutation assays-polymerase chain reaction.