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  Indian J Med Microbiol
 

Figure 1: Restriction fragment length polymorphism (RFLP) results of PCR products digested with Apa 1 enzyme. (A) Lanes 1, 2 and 4 bands were negative for A3243G mutation. In lane 3, the 494 bp products with A3243G mutation were cleaved into 364 and 130 bp fragments (mitochondrial encephalomyopathy, lactic acidosis, and stroke-like episodes DNA-positive control). L denotes 100 bp DNA ladder. (B) Lanes 1-7 indicate the number of amplified DNA and L denotes 1 kb DNA ladder. The mitochondrial genome of all the patients was amplified in 7800 bp (7.8 kb) indicating the absence of large scale deletions. (C) Lanes 1-7 indicate the number of amplified DNA and L denotes 1 kb DNA ladder. The mitochondrial genome of all the patients was amplified in 9300 bp (9.3 kb) indicating the absence of large scale deletions.

Figure 1: Restriction fragment length polymorphism (RFLP) results of PCR products digested with <i>Apa</i> 1 enzyme. (<b>A</b>) Lanes 1, 2 and 4 bands were negative for A3243G mutation. In lane 3, the 494 bp products with A3243G mutation were cleaved into 364 and 130 bp fragments (mitochondrial encephalomyopathy, lactic acidosis, and stroke-like episodes DNA-positive control). L denotes 100 bp DNA ladder. (<b>B</b>) Lanes 1-7 indicate the number of amplified DNA and L denotes 1 kb DNA ladder. The mitochondrial genome of all the patients was amplified in 7800 bp (7.8 kb) indicating the absence of large scale deletions. (<b>C</b>) Lanes 1-7 indicate the number of amplified DNA and L denotes 1 kb DNA ladder. The mitochondrial genome of all the patients was amplified in 9300 bp (9.3 kb) indicating the absence of large scale deletions.