Figure 2: Amino acids increase adiponectin mRNA expression and protein levels. (A) Quantitative PCR for adiponectin (APN) mRNA after amino acid exposure in cultured adipocytes with and without transient knockdown of adiponectin si1 10 nm. (B) Quantification of adiponectin by ELISA showed increase by 4-13-folds after amino acid exposure whereas the adiponectin si1 showed a decreased protein expression even in the presence of amino acid. *P <0.05 compared to cells treated with amino acid and without amino acids. P <0.05 compared to cells treated with and without siRNA in the presence of amino acids.