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  Indian J Med Microbiol
 

Figure 1: Optimization of adiponectin (APN) siRNA quantitative real-time PCR for APN mRNA. Adiponectin si1 10 nm at 2 (P≤0.001) and 4 h (P≤0.001) significantly decreased APN mRNA by 69 and 56 per cent whereas APN si2 100 nm (P≤0.001) and 10 nm (P <0.05) at 4 h decreased expression by 50 and 30 per cent. The maximum decrease of adiponectin mRNA was observed in APN si1 at 10 nm concentration for 2 h. *Significance (P <0.05) compared to control without siRNA. Ssi, Scrambled SiRNA.

Figure 1: Optimization of adiponectin (APN) siRNA quantitative real-time PCR for APN mRNA. Adiponectin si1 10 nm at 2 (<i>P</i>≤0.001) and 4 h (<i>P</i>≤0.001) significantly decreased APN mRNA by 69 and 56 per cent whereas APN si2 100 nm (<i>P</i>≤0.001) and 10 nm (<i>P</i> <0.05) at 4 h decreased expression by 50 and 30 per cent. The maximum decrease of adiponectin mRNA was observed in APN si1 at 10 nm concentration for 2 h. <sup>*</sup>Significance (<i>P</i> <0.05) compared to control without siRNA. Ssi, Scrambled SiRNA.