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  Indian J Med Microbiol
 

Figure 6: (A) CXCR4 expression and receptor downregulation in stromal cell-derived factor 1(SDF1) -CXCR4 signalling in haematopoietic stem and progenitor cells (HSPC). Ex vivo expanded cells stained with CXCR4 antibody - FITC conjugate. Cells were analyzed by flow cytometry. Histogram overlay of cells from unstimulated HSPC, GF HSPC and GF+SDF1 HSPC (representative of 5 experiments each). For each test, isotype antibody served as control; % expression and median fluorescence intensity (MFI) for each set are shown at right side of histogram. (B) CXCR4 expression and receptor downregulation in SDF1-CXCR4 signalling in HSPC. Flow cytometric analysis of bone marrow cells derived from mice transplanted with expanded HSPC were stained with CXCR4 antibody - FITC conjugate. Cells were analyzed by flow cytometry. Histogram overlay of cells from unstimulated HSPC, GF HSPC and GF+SDF1 HSPC (n=5). For each test, isotype antibody served as control; % expression and median fluorescence intensity for each set are shown at the right side of histogram.

Figure 6: (<b>A</b>) CXCR4 expression and receptor downregulation in stromal cell-derived factor 1(SDF1) -CXCR4 signalling in haematopoietic stem and progenitor cells (HSPC). <i>Ex vivo</i> expanded cells stained with CXCR4 antibody - FITC conjugate. Cells were analyzed by flow cytometry. Histogram overlay of cells from unstimulated HSPC, GF HSPC and GF+SDF1 HSPC (representative of 5 experiments each). For each test, isotype antibody served as control; % expression and median fluorescence intensity (MFI) for each set are shown at right side of histogram. (<b>B</b>) CXCR4 expression and receptor downregulation in SDF1-CXCR4 signalling in HSPC. Flow cytometric analysis of bone marrow cells derived from mice transplanted with expanded HSPC were stained with CXCR4 antibody - FITC conjugate. Cells were analyzed by flow cytometry. Histogram overlay of cells from unstimulated HSPC, GF HSPC and GF+SDF1 HSPC (n=5). For each test, isotype antibody served as control; % expression and median fluorescence intensity for each set are shown at the right side of histogram.