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  Indian J Med Microbiol
 

Figure 5: Colocalization of lipid grafts and CD44 in stromal cell-derived factor 1-CXCR4 signalling in haematopoietic stem and progenitor cells (HSPC). Ex vivo expanded cells were labelled with PKH-26 lipid dye. Later, these were stained with fluorescein-isothiocyanate-conjugated antibody against CD44 and analyzed by laser confocal microscopy. CD44 is colocalized in lipid rafts and is evenly distributed in unstimulated HSPC and GF HSPC. CD44 gets aggregated in lipid rafts upon stromal cell-derived factor 1 (SDF1) stimulation. Upon SDF1, stimulation cells become chemotactic and acquire migratory phenotype.

Figure 5: Colocalization of lipid grafts and CD44 in stromal cell-derived factor 1-CXCR4 signalling in haematopoietic stem and progenitor cells (HSPC). <i>Ex vivo</i> expanded cells were labelled with PKH-26 lipid dye. Later, these were stained with fluorescein-isothiocyanate-conjugated antibody against CD44 and analyzed by laser confocal microscopy. CD44 is colocalized in lipid rafts and is evenly distributed in unstimulated HSPC and GF HSPC. CD44 gets aggregated in lipid rafts upon stromal cell-derived factor 1 (SDF1) stimulation. Upon SDF1, stimulation cells become chemotactic and acquire migratory phenotype.