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  Indian J Med Microbiol
 

Figure 4: Gene expression profiling of growth factor-stimulated haematopoietic stem and progenitor cells cultures versus growth factor + stromal cell-derived factor 1 (GF+SDF1) stimulated haematopoietic stem and progenitor cells (HSPC) cultures. (A) Mean fold change for upregulated genes of homing from NCBI list in GF+ SDF1 stimulated HSPC over GF HSPC (n=2). Fold change depicted as 0.6 corresponds to 1.5 times increase of gene expression. (B) Mean fold change for genes for transcription factors involved in stem cell self-renewal, maintenance, organ morphogenesis and haematopoiesis (from NCBI list) in GF+SDF1 HSPC over GF HSPC cultures (n=2). Fold change depicted as 0.6 corresponds to 1.5 times increase of gene expression. (C) Heat maps of differential expression of genes involved in stem cell homing (left panel) and transcription factors for self renewal/ stemness (right panel) in PBSC harvest versus PBL from whole blood, GF+SDF1 stimulated HSPC versus cells from PBSC harvest and GF+SDF1 stimulated HSPC over GF-stimulated HSPC.

Figure 4: Gene expression profiling of growth factor-stimulated haematopoietic stem and progenitor cells cultures versus growth factor + stromal cell-derived factor 1 (GF+SDF1) stimulated haematopoietic stem and progenitor cells (HSPC) cultures. (<b>A</b>) Mean fold change for upregulated genes of homing from NCBI list in GF+ SDF1 stimulated HSPC over GF HSPC (n=2). Fold change depicted as 0.6 corresponds to 1.5 times increase of gene expression. (<b>B</b>) Mean fold change for genes for transcription factors involved in stem cell self-renewal, maintenance, organ morphogenesis and haematopoiesis (from NCBI list) in GF+SDF1 HSPC over GF HSPC cultures (n=2). Fold change depicted as 0.6 corresponds to 1.5 times increase of gene expression. (<b>C</b>) Heat maps of differential expression of genes involved in stem cell homing (left panel) and transcription factors for self renewal/ stemness (right panel) in PBSC harvest versus PBL from whole blood, GF+SDF1 stimulated HSPC versus cells from PBSC harvest and GF+SDF1 stimulated HSPC over GF-stimulated HSPC.