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  Indian J Med Microbiol
 

Figure 3: Assessment of in vivo engraftment potential of ex vivo expanded haematopoietic stem and progenitor cells in mice. (A) Microscopic images (×10) of platelets stained at six-week engraftment. Drop of blood from tail vein was smeared and slides were stained with May-Grunwald-Giemsa stain. Left panel depicts platelets in mouse transplanted with unstimulated haematopoietic stem and progenitor cells while right panel exhibits platelets in mouse transplanted with growth factor+stromal cell-derived factor 1 (GF+SDF1) stimulated haematopoietic stem and progenitor cells. Yellow arrow in both panels denotes location of platelets. (B) Assessment of in vivo engraftment potential of ex vivo expanded haematopoietic stem and progenitor cells in mice. Mean values (4 experiments) of relative unit area ×103 μm2 occupied by platelets in peripheral blood of mice. P values obtained by paired t test. (C) Assessment of in vivo engraftment potential of ex vivo expanded haematopoietic stem and progenitor cells in mice. Per cent human CD45+ cells in bone marrow of mice transplanted with no haematopoietic stem and progenitor cells, unstimulated haematopoietic stem and progenitor cells (HSPC), growth factor-stimulated haematopoietic stem (GF HSPC) and progenitor cells and growth factor + stromal cell-derived factor 1 stimulated haematopoietic stem and progenitor cells (GF+ SDF1 HSPC). This represents box plot of engraftment data (n=6 in each group). (D) Assessment of in vivo engraftment potential of ex vivo expanded haematopoietic stem and progenitor cells in mice. Top subpanel, confocal microscopic view, middle subpanel, 3D plot, and bottom subpanel, intensity plot of human CD45+ cells stained with fluorescein-isothiocyanate (FITC) in bone marrow of transplanted mice.

Figure 3: Assessment of <i>in vivo</i> engraftment potential of <i>ex vivo</i> expanded haematopoietic stem and progenitor cells in mice. (<b>A</b>) Microscopic images (×10) of platelets stained at six-week engraftment. Drop of blood from tail vein was smeared and slides were stained with May-Grunwald-Giemsa stain. Left panel depicts platelets in mouse transplanted with unstimulated haematopoietic stem and progenitor cells while right panel exhibits platelets in mouse transplanted with growth factor+stromal cell-derived factor 1 (GF+SDF1) stimulated haematopoietic stem and progenitor cells. Yellow arrow in both panels denotes location of platelets. (<b>B</b>) Assessment of <i>in vivo</i> engraftment potential of <i>ex vivo</i> expanded haematopoietic stem and progenitor cells in mice. Mean values (4 experiments) of relative unit area ×10<sup>3</sup> μm<sup>2</sup> occupied by platelets in peripheral blood of mice. <i>P</i> values obtained by paired <i>t</i> test. (<b>C</b>) Assessment of <i>in vivo</i> engraftment potential of <i>ex vivo</i> expanded haematopoietic stem and progenitor cells in mice. Per cent human CD45+ cells in bone marrow of mice transplanted with no haematopoietic stem and progenitor cells, unstimulated haematopoietic stem and progenitor cells (HSPC), growth factor-stimulated haematopoietic stem (GF HSPC) and progenitor cells and growth factor + stromal cell-derived factor 1 stimulated haematopoietic stem and progenitor cells (GF+ SDF1 HSPC). This represents box plot of engraftment data (n=6 in each group). (<b>D</b>) Assessment of <i>in vivo</i> engraftment potential of <i>ex vivo</i> expanded haematopoietic stem and progenitor cells in mice. Top subpanel, confocal microscopic view, middle subpanel, 3D plot, and bottom subpanel, intensity plot of human CD45+ cells stained with fluorescein-isothiocyanate (FITC) in bone marrow of transplanted mice.