Figure 2: Stem cell progenitor assessment by colony formation assay (CFA). (A) Representative images of colony forming unit-erythrocyte (cfu-E), blast-forming unit-erythrocyte ( bfu-E), colony-forming unit granulocyte macrophage (cfu-GM) (neutrophils), colony-forming unit granulocyte macrophage (cfu-GM) (eosinophils), cfu-granulocyte erythrocyte monocyte, megakaryocyte (cfu-GEMM), cfu-M colonies observed in CFA of enriched haematopoietic stem and progenitor cells under confocal microscope. Colonies were photographed after 14 days differentiation on methocult medium. (B) Stem cell progenitor assessment by colony formation assay. Total colony unit area (mean values) occupied by colonies generated by primitive and progenitor haematopoietic stem cells in ex vivo expanded haematopoietic stem and progenitor cells cultures (n=5).