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  Indian J Med Microbiol
 

Figure. 3. Reduced SMAR1 level during colonic inflammation mediated by Th1 and Th17 cells. (A) Frequencies of CD4+ T cells from the spleen and mesenteric lymph nodes (MLNs) of WT and SCID mice evaluated by flow cytometry. (B) Representative colon gross anatomy of WT and WT CD4+CD45RBhi T cells transferred to SCID mice (WT → SCID). (C) H & E staining of colon sections from the mice in B of a similar region (arrow) with 10× magnification. (D) Sorted CD4+CD45RBhi T cells from WT mice were transferred in to 6-wk old SCID mice, and the weight of the mice assessed weekly. *P<</i> 0.002; determined by unpaired student t test. Values are mean ± SEM (n=6). (E) Frequencies of IFNã+ and IL-17+ cells among total T cells isolated from colon lamina propria (LP) sections from the mice in B. Values are mean ± SEM (n=6). (F) Frequencies of CD4+SMAR1+ cells among total colon LP cells after 1st, 3rd, 5th and 7th week isolated from colon sections of WT CD4+CD45RBhi T cells transferred to SCID mice. Values are mean ± SEM (n=6). (G) Total protein lysate, prepared from the colonic LP T cells from the treated mice as described in (F) , analyzed for the pSTAT3 and SMAR1 expression by Western blotting showing decreased SMAR1 and increased pSTAT expression. â-actin was used as a control. Data representative of three independent experiments each with four mice per group.

Figure. 3. Reduced SMAR1 level during colonic inflammation mediated by Th1 and Th17 cells. (A) Frequencies of CD4+ T cells from the spleen and mesenteric lymph nodes (MLNs) of WT and SCID mice evaluated by flow cytometry. (B) Representative colon gross anatomy of WT and WT CD4+CD45RBhi T cells transferred to SCID mice (WT → SCID). (C) H & E staining of colon sections from the mice in B of a similar region (arrow) with 10× magnification. (D) Sorted CD4+CD45RBhi T cells from WT mice were transferred in to 6-wk old SCID mice, and the weight of the mice assessed weekly. <i>*P</i><</i> 0.002; determined by unpaired student t test. Values are mean ± SEM (n=6). (E) Frequencies of IFNã+ and IL-17+ cells among total T cells isolated from colon lamina propria (LP) sections from the mice in B. Values are mean ± SEM (n=6). (F) Frequencies of CD4+SMAR1+ cells among total colon LP cells after 1st, 3rd, 5th and 7th week isolated from colon sections of WT CD4+CD45RBhi T cells transferred to SCID mice. Values are mean ± SEM (n=6). (G) Total protein lysate, prepared from the colonic LP T cells from the treated mice as described in (F) , analyzed for the pSTAT3 and SMAR1 expression by Western blotting showing decreased SMAR1 and increased pSTAT expression. â-actin was used as a control. Data representative of three independent experiments each with four mice per group.