Figure 5. Discriminatory restriction pattern of representative MAP isolates of Indian and non-Indian origin using IS1311 PCR locus 2-REA method. Lane 1 Marker (50bp); Lane 2: Negative control, Lane 3: Undigested PCR product (~425bp); Lane 4: isolates no. W213 (Cattle type of USA origin); Lane 5: isolates no. 22G (Sheep type of Spain origin); Lane 6: isolates no. MAP B1 (Bison type of Canada origin); Lane 7: isolates no. MAP 16 (Bison type of Indian origin); Lane 8: isolates no. MAP 18 (Bison type of Indian origin).