Figure 3: Confocal microscopy showing effect of leptin on co-localization of LDL receptor and insulin receptor. Insulin receptor was stained with FITC (Fluorescein sothiocyanate; green) and LDL receptor was stained with PE (Phycoerythrin; red). The co-localization of the two receptors (green and red) was observed as yellow colour. The density of yellow colour was increased with 1nM leptin. This showed increased co-localization with leptin.