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  Indian J Med Microbiol
 

Figure 1A: Expression of LDL receptor (LDLR) protein in HepG2 cells with increasing concentration of leptin. The figure shows LDLR protein expression by western blotting. Leptin showed inhibition of LDL receptor expression. Each bar in the graph represents mean ± SD [(% Integrated density value (IDV) normalized against respective β-actin expression]; n=3; statistical analysis was carried out by one-way ANOVAfollowed by Dunnette's multiple comparison; **P <0.01. B. Expression of LDL receptor mRNAin HepG2 cells with increasing concentration of leptin. The figure depicts the LDLR transcript expression by semi-quantitative RT-PCR. LDLR mRNAwas inhibited in a dose dependent manner with increasing leptin concentration. Data represented as mean ± SD (%IDV normalized against respective β-actin expression); n=3; statistical analysis was carried out by one-way ANOVAfollowed by Dunnette's multiple comparison; **P <0.01. C. Expression of SREBP2 in HepG2 cells with various concentration of leptin in the culture medium. The figure represents the expression of SREBP2 by Western blotting. Leptin inhibited the expression of SREBP2. Each bar in the graph represents mean ± SD (%IDV normalized against respective β-actin expression); n=3; statistical analysis was carried out by one-way ANOVAfollowed by Dunnette's multiple comparison; **P <0.01.

Figure 1A: Expression of LDL receptor (LDLR) protein in HepG2 cells with increasing concentration of leptin. The figure shows LDLR protein expression by western blotting. Leptin showed inhibition of LDL receptor expression. Each bar in the graph represents mean ± SD [(% Integrated density value (IDV) normalized against respective β-actin expression]; n=3; statistical analysis was carried out by one-way ANOVAfollowed by Dunnette's multiple comparison; **<i>P</i> <0.01. B. Expression of LDL receptor mRNAin HepG2 cells with increasing concentration of leptin. The figure depicts the LDLR transcript expression by semi-quantitative RT-PCR. LDLR mRNAwas inhibited in a dose dependent manner with increasing leptin concentration. Data represented as mean ± SD (%IDV normalized against respective β-actin expression); n=3; statistical analysis was carried out by one-way ANOVAfollowed by Dunnette's multiple comparison; **<i>P</i> <0.01. C. Expression of SREBP2 in HepG2 cells with various concentration of leptin in the culture medium. The figure represents the expression of SREBP2 by Western blotting. Leptin inhibited the expression of SREBP2. Each bar in the graph represents mean ± SD (%IDV normalized against respective β-actin expression); n=3; statistical analysis was carried out by one-way ANOVAfollowed by Dunnette's multiple comparison; **<i>P</i> <0.01.