Figure 3: Stability of oxytocin during gastric and intestinal phase of digestion: Native or reduced oxytocin in the absence (A) or presence of milk (B) was incubated 0 to 2 h with gastric fluid at 37 oC. Oxytocin in the absence (C) or presence of milk (D) was incubated with intestinal fluid with and without 10 mM phenylmethyl sulphonyl fluoride (PMSF) for 0 to 1h. Aliquots (100 μl) of these samples were analyzed by HPLC. The % oxytocin was calculated assuming the peak area of oxytocin at 0 h as 100%. The bars represent mean±SD (n=6) and bars that do not share common superscript differ significantly (P<0.05).