Figure 4: In vitro differentiation of Lin- BMCs into megalin, E-cadherin and CK-19 expressing epithelial cells. Lin- BMCs were cultured for 7 days. The cultured cells were stained with anti-CD45, anti-megalin, anti-CK19, anti-vimentin, and anti-E-cadherin antibodies. (A) Fresh Lin- BMCs stained with CD45/PE staining; (B-E): Lin- BMCs cultured in normal serum supplemented medium (negative control), (B) Adhered cells in bright field, (C) Megalin, (D) CK-19, (E) Vimentin; (F, G): Lin- BMCs were cultured in differentiating medium, (F) Adhered cells in bright field, (G) Co-stained for megalin and CD45 (enlarged images of cells in left two boxes are shown in the right); (H-J): Lin- BMCs were cultured in differentiating medium, (H) Megalin, (I) Vimentin, (J) Merge image of H & I; (K-M): Lin- BMCs were cultured in differentiating medium, K. Megalin, (L) E-cadherin, (M) Merge image of K & L; (N-P): Lin- BMCs were cultured in differentiating medium, (N) Megalin, (O) CK-19, (P) Merge image of N & O; (Q-R): Primary kidney cells (positive control), (Q) Megalin, (R) CK-19, (S) Merge of Q & R. Not all cells show the expression of megalin and CK-19 due to crude preparation of the primary cells. Inset show magnified images of cells (differentiated tubular epithelial and primary renal epithelial) co-expressing megalin and CK-19. Nuclei were stained blue with DAPI. Number of experiment = 3. Magnification: 200×.