Figure 3: Flow cytometric analysis of eGFP+, GFP+Megalin+, and GFP+vWF+ cells. Megalin (Meg) and vWF antibody stained cells from cortex and outer medullary regions (forced-engrafted approach) were analyzed by flowcytometer after 1 month of transplantation. (A) Representative dot-plots for eGFP+, GFP+Meg+, and GFP+vWF+ cells are shown. The analysis is based on 'R1' gated cells in SSC/FSC plot. (B) Percentage engraftment of eGFP+ cells and their differentiation into GFP+Meg+ and GFP+vWF+ cells determined from the dot-plots. n = 4. *P<0.01 with respect to GFP+vWF+ cells.