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  Indian J Med Microbiol
 

Figure 2: Expression of megalin and vWF in the donor-derived cells (induced-engraftment approach). eGFP chimeric mice received single injection of HgCl2. Serial kidney cryosections (5 μm) were stained with anti-GFP, anti-megalin, and anti-vWF antibodies. The representative photomicrographs after 1 month of HgCl2 treatment are shown. (A) eGFP-expressing cells are engrafted in the interstitial space, but do not express megalin. (C) Magnified view of a section distinctly shows that the donor cells (long white arrow) are not expressing megalin. The host tubular epithelial cells (short white arrow) are expressing megalin (red). (E) Co-expression of eGFP and vWF in non-tubular cells. Expression of megalin and vWF in the donor-derived cells (forced-engraftment approach). After 48 h of HgCl2 treatment, each mouse received tail vein injection of 5 x 106 eGFP-expressing BMCs. One month after transplantation mice were sacrificed and kidney tissues were analyzed. Serial kidney cryosections (5 μm) were stained with anti-GFP, anti-megalin, and anti-vWF antibodies. (B) eGFP-expressing cells are mostly engrafted on the tubules, many of them expressed megalin. (D) Magnified view of a section distinctly shows that donor cells (white long arrow) expressing megalin (red) on the apical surface of the tubular epithelial cells. (F) Photomicrographs show eGFP and vWF are not expressed by the same cells. Number of mice (n) = 4 for each group. Magnification: 400× (megalin); 200× (vWF).

Figure 2: Expression of megalin and vWF in the donor-derived cells (induced-engraftment approach). eGFP chimeric mice received single injection of HgCl2. Serial kidney cryosections (5 μm) were stained with anti-GFP, anti-megalin, and anti-vWF antibodies. The representative photomicrographs after 1 month of HgCl2 treatment are shown. <b>(A)</b> eGFP-expressing cells are engrafted in the interstitial space, but do not express megalin. <b>(C)</b> Magnified view of a section distinctly shows that the donor cells (long white arrow) are not expressing megalin. The host tubular epithelial cells (short white arrow) are expressing megalin (red). <b>(E)</b> Co-expression of eGFP and vWF in non-tubular cells. Expression of megalin and vWF in the donor-derived cells (forced-engraftment approach). After 48 h of HgCl2 treatment, each mouse received tail vein injection of 5 x 106 eGFP-expressing BMCs. One month after transplantation mice were sacrificed and kidney tissues were analyzed. Serial kidney cryosections (5 μm) were stained with anti-GFP, anti-megalin, and anti-vWF antibodies. <b>(B)</b> eGFP-expressing cells are mostly engrafted on the tubules, many of them expressed megalin. <b>(D)</b> Magnified view of a section distinctly shows that donor cells (white long arrow) expressing megalin (red) on the apical surface of the tubular epithelial cells. <b>(F)</b> Photomicrographs show eGFP and vWF are not expressed by the same cells. Number of mice (n) = 4 for each group. Magnification: 400× (megalin); 200× (vWF).