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  Indian J Med Microbiol
 

Figure 3: PCR amplification of 340 bp region of 38 kDa gene and 245 region of IS6110 of M. tuberculosis using different ratios of concentrations of KD1 & KD2 and INS1 & INS2 primers. Lane M- DNA ladder (a) Lanes 1, 2, 3 and 4 - KD1/2 and INS1/2 in ratios 0.5:0.25, 0.25:0.25, 0.25:0.5 and 0.5:0.15 (all in μM concentration) respectively with template MTB DNA concentration of 10pg. (b) Lanes 1, 2 and 3- template DNA concentration of 1 pg, 100 fg and 10 fg, respectively with KD1/2 and INS1/2 in ratios 0.5:0.25 in μM concentration per reaction.

Figure 3: PCR amplification of 340 bp region of 38 kDa gene and 245 region of <i>IS6110</i> of <i>M. tuberculosis</i> using different ratios of concentrations of KD1 & KD2 and INS1 & INS2 primers. Lane M- DNA ladder <b>(a)</b> Lanes 1, 2, 3 and 4 - KD1/2 and INS1/2 in ratios 0.5:0.25, 0.25:0.25, 0.25:0.5 and 0.5:0.15 (all in μM concentration) respectively with template MTB DNA concentration of 10pg. <b>(b)</b> Lanes 1, 2 and 3- template DNA concentration of 1 pg, 100 fg and 10 fg, respectively with KD1/2 and INS1/2 in ratios 0.5:0.25 in μM concentration per reaction.