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  Indian J Med Microbiol
 

Figure 1(a): JAK2V617F mutation by Allele specific PCR . Lane M, 100-bp ladder; lanes 1-5, samples from patients, lanes 2, 3 and 5 show mutant band (203 bp) carried by the patients. Lanes 1 and 4 show a single band (364bp) which is a wild type band and acts as an internal PCR control, lane B is the negative control. Arrows indicate the product of amplification. (b) Restriction enzyme analysis of JAK2V617F mutation. Lanes 1-5, samples from patients, lane B is the negative control, lanes 1, 2 and 4 show homozygous mutant band at 460 bp as assessed by BsaXI restriction enzyme. Lanes 3 and 5 show the wild allele which was digested into 241, 189 and 30 bp fragments.

Figure 1(a): <i>JAK2V617F</i> mutation by Allele specific PCR <b>.</b> Lane M, 100-bp ladder; lanes 1-5, samples from patients, lanes 2, 3 and 5 show mutant band (203 bp) carried by the patients. Lanes 1 and 4 show a single band (364bp) which is a wild type band and acts as an internal PCR control, lane B is the negative control. Arrows indicate the product of amplification. <b>(b)</b> Restriction enzyme analysis of <i>JAK2V617F</i> mutation. Lanes 1-5, samples from patients, lane B is the negative control, lanes 1, 2 and 4 show homozygous mutant band at 460 bp as assessed by BsaXI restriction enzyme. Lanes 3 and 5 show the wild allele which was digested into 241, 189 and 30 bp fragments.