Figure 4a and b. Western blot of total ERK1/2 (4a) and pERK1/2 (4b) in untreated, hCG treated and LH treated cultures at different time points. hCG and LH treated cultures show similar expression whereas there is no expression of pERK1/2 in untreated cultures at 0 and 40 min (L-ladder, C-positive control). Anti-â actin antibody was used to ensure equal loading. Figure 4c & d. Flowcytometric analysis of ERK after short term stimulation with LH or hCG for 30 min. hCG and LH treated cultures show similar increase in total ERK (4c) and phosphorylated ERK (4d) expression. Values are mean ± SEM of 3 observations.