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  Indian J Med Microbiol
 

Figure 3a: LH and hCG increased PKA activity in a time dependent manner in short term cultures of granulosa cells (day 5). Data expressed as mean ± SEM (n=3). Increase in PKA activity is seen after stimulation with LH as well as hCG, with maximum elevation seen in LH treated cultures. **P<0.001. b. LH and hCG increased PKA activity in a time dependent manner in long term cultures of granulosa cells (2 wk). Data expressed as mean ± SEM (n=3). *P<0.05 compared to LH treated cultures in a time dependent manner.c. LH and hCG stress increased PKC activity in a time dependent manner in short term cultures of granulosa cells (day 5). Data is expressed as mean±SEM. Increase in PKC activity in a time dependent manner is seen after stimulation with LH or hCG (*P<0.05). d. PKC activity is more in untreated cells as compared to hCG treated and LH treated cultures after long term stimulation (2 wk). Data expressed as mean ± SEM. Increase in PKC activity is maximum in untreated cells (P=ns).

Figure 3a: LH and hCG increased PKA activity in a time dependent manner in short term cultures of granulosa cells (day 5). Data expressed as mean ± SEM (n=3). Increase in PKA activity is seen after stimulation with LH as well as hCG, with maximum elevation seen in LH treated cultures. **<i>P</i><0.001. b. LH and hCG increased PKA activity in a time dependent manner in long term cultures of granulosa cells (2 wk). Data expressed as mean ± SEM (n=3). *<i>P</i><0.05 compared to LH treated cultures in a time dependent manner.c. LH and hCG stress increased PKC activity in a time dependent manner in short term cultures of granulosa cells (day 5). Data is expressed as mean±SEM. Increase in PKC activity in a time dependent manner is seen after stimulation with LH or hCG (*<i>P</i><0.05). d. PKC activity is more in untreated cells as compared to hCG treated and LH treated cultures after long term stimulation (2 wk). Data expressed as mean ± SEM. Increase in PKC activity is maximum in untreated cells (<i>P</i>=ns).