Year : 2018 | Volume
: 148 | Issue : 6 | Page : 713--720
Molecular genotyping of clinically important blood group antigens in patients with thalassaemia
Swati Kulkarni1, Bhavika Choudhary1, Harita Gogri1, Shashikant Patil2, Mamta Manglani3, Ratna Sharma3, Manisha Madkaikar1
1 Department of Transfusion Medicine, ICMR-National Institute of Immunohaematology, KEM Hospital Campus, Mumbai, India
2 Arpan Blood Bank, Nashik, India
3 Pediatric Hematology-Oncology & BMT Centre, Lokmanya Tilak Municipal General Hospital, Mumbai, India
Background & objectives: In multitransfused thalassaemic patients, haemagglutination fails to phenotype the patient's blood group antigens due to the presence of donor-derived erythrocytes. DNA-based methods can overcome the limitations of haemagglutination and can be used to determine the correct antigen profile of these patients. This will facilitate the procurement of antigen-matched blood for transfusion to multitransfused patients. Thus, the aim of this study was to compare the serological phenotyping of common and clinically important antigens of Rh, Duffy, Kell, Kidd and MNS blood group systems with molecular genotyping amongst multitransfused thalassaemic patients.
Methods: Blood samples from 200 patients with thalassaemia and 100 'O' group regular blood donors were tested using standard serological techniques and polymerase chain reaction-based methods for common antigens/alleles (C, c, D, E, e, Fya, Fyb, Jka, Jkb, K, k, M, N, S, s).
Results: Genotyping and phenotyping results were discordant in 77 per cent of thalassaemic patients for five pairs of antithetical antigens of Rh, Duffy, Kell and Kidd blood group systems. In the MNS blood group system, 59.1 per cent of patients showed discrepancy. The rate of alloimmunization among thalassaemics was 7.5 per cent.
Interpretation & conclusions: Molecular genotyping enabled the determination of the actual antigen profile in multitransfused thalassaemia patients. This would help reduce the problem of alloimmunization in such patients and would also aid in the better management of transfusion therapy.
Dr Swati Kulkarni
Department of Transfusion Medicine, ICMR-National Institute of Immunohaematology, 13th Floor, New Multi-Storeyed Building, KEM Hospital Campus, Parel, Mumbai 400 012, Maharashtra
|How to cite this article:|
Kulkarni S, Choudhary B, Gogri H, Patil S, Manglani M, Sharma R, Madkaikar M. Molecular genotyping of clinically important blood group antigens in patients with thalassaemia.Indian J Med Res 2018;148:713-720
|How to cite this URL:|
Kulkarni S, Choudhary B, Gogri H, Patil S, Manglani M, Sharma R, Madkaikar M. Molecular genotyping of clinically important blood group antigens in patients with thalassaemia. Indian J Med Res [serial online] 2018 [cited 2020 Sep 24 ];148:713-720
Available from: http://www.ijmr.org.in/article.asp?issn=0971-5916;year=2018;volume=148;issue=6;spage=713;epage=720;aulast=Kulkarni;type=0